Fig. 6

Down-regulated miR-146a expression with increased NETs and apoptosis formation in the mouse DAH lungs. a Right, representative gross and histopathological photographs in the mouse lungs with no and complete hemorrhage. Left, hemorrhage frequencies in saline- and pristane-injected mice. Scale bar = 100 µm, magnification × 100. b Neutrophil, RBC numbers, Hct and Hb levels in saline- and pristane-injected mice. c MiR-146a pulmonary (left) and splenic (right) levels on day 0, 4, 9 and 14 from saline- and pristane-injected mice. d IL-6 (left) and IL-8 (right) pulmonary levels on day 0, 4, 9 and 14 from saline- and pristane-injected mice. e Representative immunoblot assay (right) with signal intensity quantitation (left) of pulmonary CitH3, PAD4, HMGB1, TRAF6, IRAK1 and β-actin expression from saline- and pristane-injected mice. f Representative CitH3 IF staining (green) in lung tissues from saline- and pristane-injected mice. Cell nuclei counterstained with Hoechst 33,258 (blue). Scale bar = 12.5 µm, magnification × 800. g Left, representative TUNEL IF staining (green) in lung tissues from saline- and pristane-injected mice. Right, quantification of TUNEL-positive cell numbers in lung tissues. Cell nuclei counterstained with DAPI (blue). Scale bar = 25 µm, magnification, × 400. Values are mean ± SEM. Horizontal lines are mean values. Mouse numbers per group, 10 in a, 4 or 5 in b, 5 in c, d, 3 in e, 5 in g. All results in this figure were representative of 2 independent experiments with similar findings. * p < 0.05, ** p < 0.01, *** p < 0.001