Fig. 2

Intrarectal administration of LGp40-overexpressing Clear coli alleviated HDM-induced asthma in mice. a Experimental scheme of Protocol 1. SDS-PAGE showed the quantities of LGp40 derived from IPTG-non-induced and IPTG-induced Clear coli. b Total IgE serum concentrations were measured using ELISA (n = 10 mice, *p < 0.05 and **p < 0.01, one-way ANOVA with Bonferroni multiple comparison test). c Rrs for increasing dosages of aerosolized methacholine were measured using the flexiVent FX system (n = 10 mice, *p < 0.05 and **p < 0.01, two-way ANOVA with Bonferroni multiple comparison test). d Total cell, eosinophil, neutrophil, lymphocyte, and macrophage counts in the BALF were determined (n = 10 mice, *p < 0.05 and **p < 0.01, one-way ANOVA with Bonferroni multiple comparison test). e TARC production in BALF was determined using ELISA (n = 10 mice, *p < 0.05, one-way ANOVA with Bonferroni multiple comparison test). f Lung histology. Lung sections were stained with hematoxylin and eosin (H&E; scale bar = 200 μm). Results comprise pooled data from two independent experiments