Fig. 2

Tetrandrine reduces Iba-1 (gliosis) and AT8 (phosphorylated tau) signals in Thy1-hTau.P301S mice. Micrographs showing representative immunofluorescence staining of gliosis and phosphor-tau in hippocampal regions of control wild-type C57 (C57 WT) or Thy1-hTau.P301S mice treated with saline (Tg-Vehicle) or different concentrations of tetrandrine (TET; 2.5 mg/kg, 5 mg/kg, and 10 mg/kg, ip injection every two days from 2 months old to 4 months old). After the treatment, brain slices were immuno-probed with Iba-1 and AT8 antibodies to reveal gliosis and hyperphosphorylated tau. The bottom panel shows the magnified region in the white boxes in the AT8 immunostaining panels. The arrowheads of the magnified micrographs depict neurons with hyperphosphorylated tau. Quantifications of Iba1 and AT8 levels are shown in the bar charts. Data are summarized as the mean ± SEM from 8 mice, with 24 images analysed in each group. *Indicates p < 0.05 compared with C57 WT or vehicle-treated tau mice control