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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Hepatitis B virus suppresses complement C9 synthesis by limiting the availability of transcription factor USF-1 and inhibits formation of membrane attack complex: implications in disease pathogenesis

Fig. 4

HBX limits USF-1 availability for C9 transcription by hypermethylation of USF-1 promoter. a CpG island prediction in USF-1 promoter using Methprimer online software. b Relative expression of USF1 and C9 in HBx transfected Huh7 cells with or without DNA methylation inhibitor 5-aza-2′-deoxycytidine (5 μM). c Relative expression of DNMT3A in Huh7 cells transfected with HBX-expressing plasmid and empty vector. d Schematic representation of CpG dinucleotides at USF-1 promoter in Huh7 cells transfected with HBX-expressing plasmid and empty vector. The horizontal line represents the sequencing result of one clone, and the vertical line represents each individual CpG sites. The open circles represent unmethylated cytosine and the closed circles represent methylated cytosine. The percentages of methylated CpGs in USF-1 promoter in case of HBx and empty vector transfected Huh7 cells were calculated and compared. Values represented as mean ± SD. Paired t tests were performed for comparing paired groups. **p < 0.005, ***p < 0.0001

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Journal of Biomedical Science

ISSN: 1423-0127