Fig. 3

DCLK1 participates in IL-8/CXCL8 release stimulated by thrombin in A549 and BEAS-2B cells. A549 (A) and BEAS-2B (B) cells were transfected with DCLK1 siRNA or control (con siRNA) for 48 h, then treated for additional 24 h with thrombin (10 U/ml). The level of IL-8/CXCL8 release was detected using ELISA (n = 3–4, mean ± SEM, *p < 0.05 vs thrombin-stimulated group). A549 (C) and BEAS-2B (D) cells were transfected with DCLK1 siRNA or control (con siRNA) for 48 h. Immunoblotting was performed to determine the expression level of DCLK1 (n = 3, mean ± SEM, *p < 0.05 vs control). A549 (E) and BEAS-2B (F) were incubated with LRRK2-IN-1 for 30 min, and the cells were subsequently treated for additional 24 h with 10 U/ml thrombin. The level of IL-8/CXCL8 release was determined using ELISA (n = 3, mean ± SEM, *p < 0.05 vs thrombin-stimulated group)