Fig. 6
From: Secretory autophagy promotes Rab37-mediated exocytosis of tissue inhibitor of metalloproteinase 1
The effects of Sec22b on Rab37 and autophagy-mediated TIMP1 secretion and tumorigenesis in H460 cells. a The level of secreted TIMP1 in condition medium and the levels of Sec22b, LC3, and Rab37 in the cell lysate were determined by immunoblotting using specific antibodies. β-actin and Coomassie gel staining of the total proteins represent equal loading controls of the proteins in the cells and in the supernatant, respectively. b Colocalization of rhodamine-labeled Rab37 (red) and FITC-labeled LC3 (green) was investigated under starvation conditions for 24 h under a multi-photon confocal microscope. Quantification of colocalization of Rab37 and LC3 was conducted by randomly counting 30 cells. Scale bar = 10 µm. Error bars represent mean ± SD. Data were analyzed by Student’s t-test; ***p < 0.001