Fig. 5

Knockdown of Hotair reduces cell proliferation and downregulates pro-inflammatory cytokine expression in mouse MBT-2 bladder cancer cells. MBT-2 cells transduced with lentiviral vectors containing CRISPRi targeting mouse Hotair (MBT-2/CSi-Hotair-F4 and -F6) or GFP (MBT-2/CSi-GFP) were assessed for Hotair expression by RT-qPCR (a), cell proliferation by the MTS assay (b), as well as IL-6 (c), TNF-α (d), and IL-1β (e) transcripts by RT-qPCR. Values shown are mean ± SD (n = 3–5). The ratios of control cells were arbitrarily set to 100