Fig. 4

LSD1 is elevated in the motor neurons of an animal model of ALS. A Western blot analysis of LSD1 expression assessment in ALS (G93A) mice compared to WT at 90 and 120 days of age. B The densitometry analysis shows a significant increase of LSD1 and decrease of H3K4me2 protein level in G93A mice. C A scheme illustrating a work flow of animal experiments. D Immunoreactivity of LSD1 (brown) and H3K4me2 was mainly found in the nucleus of motor neurons in ventral horn of WT and G93A mice. E SD administration decreased the punctate structures of LSD1 immunoreactivity in the nucleus of motor neurons of ALS (G93A) mice. The densitometry analysis shows that LSD1 is significantly reduced by SD (bottom panel). The number of cell counting: 7 cells/group. F SD administration restored the H3K4me2 immunoreactivity in the nucleus of motor neurons of ALS (G93A) mice. An arrow indicates a motor neuron with the lowered level of H3K4me2. The densitometry analysis shows that H3K4me2 is significantly elevated by SD (bottom panel). Scale bars (white): 10 μm. The number of cell counting: 7 cells/group. Significantly different at *p < 0.05 and **p < 0.01