Fig. 4

Immune cell trafficking to the lens in the dotted ring stage of diabetic cataractogenesis. A IHC of MAGP1 to stain the zonular fibers in conventional sagittal section, and B in our new frontal eye globe approach show cells attached to the zonular fibers, and C at higher magnification. D CD45 staining in a conventional sagittal section, and E in our new frontal eye globe preparation, and F at higher magnification show immune cells in the vicinity of the ciliary bodies and zonular fibers. G H&E staining, and H frontal eye globe preparation, and I at higher magnification revealed presence of pigmented cells (arrowheads) on the surface of ciliary bodies and the zonular fibers. J IHC in lens flatmounts demonstrated CD45 positive cells on the lens capsule, K at higher magnification, and L some of which were pigmented (white arrowheads). M Histologic sections of the lens in the dotted ring stage show presence of intra-capsular cells, co-localized with TUNEL positive LECs, and N at higher magnification. Dashed line indicates position of the lens capsule. O Quantification of the migratory cells in the space between the ciliary bodies and the lens in normal, dotted ring stage, and advanced cataractous eyes (n = 3, in each group)