Fig. 1
Galectin-3 is upregulated in the BAL fluid and lungs of mice following IAV infection. Mice were intranasally (a, b) and intratracheally (c) inoculated with IAV (105 PFU/mouse) at day 0, and the BAL fluid (a, b) and lung tissue (c) were collected at different time points, respectively. a Detection of galectin-3 levels in the BAL fluid by ELISA. Values shown are mean ± SD (n = 3). b Detection of galectin-3 and the viral NP in the lung of individual mice by immunoblotting (left). Each lane represents samples from individual mice. Expression of β-actin served as the loading control. Pearson correlation analysis for the correlation of galectin-3 and viral NP levels showing the best-fit linear trend line (right). c Immunohistochemical detection (original magnification ×200; scale bar = 100 μm) (left) and quantitation of immunoreactive intensity (right) of galectin-3 with AEC (red color) as the chromogen in the lung at the recovery stage of IAV infection, with the value of normal mice arbitrarily set to 100. Values shown are mean ± SD (n = 4)