Fig. 2
Deficiency in galectin-3 reduces mouse lethality after IAV challenge and decreases susceptibility of mouse macrophages and lung fibroblasts to IAV infection. a, b Gal-3−/− and WT mice were intranasally inoculated with 105 PFU of IAV at day 0. Changes in body weights (from day 0 to day 7) expressed as the percentage of pre-infection (day 0) body weight (a) and Kaplan-Meier survival curves (b). c Detection of the viral NP in the macrophages of Gal-3−/− and WT mice infected with IAV. BMDMs were infected with IAV at MOI of 1, 5, and 10, or mock-infected for 24 h. Levels of viral NP and galectin-3 proteins in the cell lysates were examined by immunoblotting. Expression of β-actin served as the loading control. d Immunofluorescence detection (left) and quantitation of immunoreactive intensity (right) of the viral NP in lung fibroblasts of Gal-3−/− and WT mice infected with IAV. Primary lung fibroblasts from both mice were infected with IAV at an MOI of 5 for 24 h. Nuclei were stained with DAPI. Values shown are mean ± SD (n = 4)