Skip to main content
Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Upregulation of galectin-3 in influenza A virus infection promotes viral RNA synthesis through its association with viral PA protein

Fig. 7

Knockdown of galectin-3 decreases the RdRp activity of IAV. a–c Galectin-3-knockdown and vector control A549 cells were infected with IAV at an MOI of 1 for 7 h. Quantification of vRNA (a), mRNA (b), and cRNA (c) of the viral NP by RT-qPCR and normalization to GAPDH mRNA levels (n = 3). Ratios of shLacZ vector control cells were arbitrarily set to 1. d Detection of viral polymerase activity by the minireplicon assay. Galectin-3-knockdown and vector control 293T cells were cotransfected with pPolI-Luc and four expression vectors individually encoding IAV proteins (PA, PB1, PB2, and NP). At 48 h post-transfection, their luciferase activities were determined and expressed as light units/µg protein (n = 3)

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127