Fig. 2

Rrbp1-KO mice show hyporeninemic hypoaldosteronism. A–F Plasma angiotensinogen, renin, Ang-I, Ang-II, aldosterone levels, and plasma renin activity (PRA) in mice. G Western blot analysis of SGK1 protein expression in kidneys harvested from Rrbp1-WT and Rrbp1-KO mice. H Quantification of the immunoblot in (G). I mRNA levels of Scnn1a, Scnn1b, Scnn1g, and Kcnj1 in mice kidney measured using quantitative RT-PCR. Data were analyzed using the 2-ΔΔCt method with GAPDH as the reference gene (n = 28–35 per group). J Protocol for blood and urine test for mice that underwent high K⁺ intake. K–M Plasma renin, Ang-II, and aldosterone levels in mice that underwent high K⁺ intake for 48 h. N Study protocol for recording survival rate of mice that underwent high K.⁺ intake for 30 days with 0, 2.5, 10 mg/kg fludrocortisone treatment (FC) and control mice, respectively. O Kaplan–Meier survival curve and log-rank analysis of Rrbp1-KO mice rescued with 0, 2.5, 10 mg/kg fludrocortisone treatment (FC) and control mice, respectively. WT, wild-type; KO, knock-out. Data in A–F, I, and K–M were analyzed with an unpaired, two-tailed Student’s t-test; data in H were analyzed using the Mann–Whitney test. Data are represented as mean ± SEM. PRA, plasma renin activity; RFU, relative fluorescence units; ns, no significance; *P < 0.05; ** P < 0.01; ***P < 0.001; **** P < 0.0001