Table 1 Bacterial strains and plasmids used in this study

Strains

 SMC1204-109 (109)

K. pneumoniae ST23, Serotype K1, clinical isolate

[12]

 SMC1207-200 (200)

K. pneumoniae ST23, Serotype K1, clinical isolate

[12]

 SMC1204-109-IR (109-IR)

Tigecycline-resistant mutant developed from 109

[12]

 SMC1207-200-IR (200-IR)

Tigecycline-resistant mutant developed from 200

[12]

 109/ompR

109, ompR-overexpressed by IPTG

This study

 200/ompR

200, ompR-overexpressed by IPTG

This study

 109-IR∆ompR

109-IR, ompR inactivated

This study

 200-IR∆ompR

200-IR, ompR inactivated

This study

 109-IR∆ompR-C

109-IR∆ompR, ompR-complemented

This study

 200-IR∆ompR-C

200-IR∆ompR, ompR-complemented

This study

Plasmids

 pUHE21-2lacI^q

P_lac rep_pMBI Amp^R lacI^q

[25]

 pDK4

rep_R6Kγ Amp^R FRT Km^R FRT

[13]

 pKD46

rep_pSC101(Ts) Amp^R P_araBAD γ β exo

[13]

 pCVD442

rep_R6Kγ Gm^R

[26]

 pHK251

pUHE21-2lacI^q with an insertion of Gm^R cassette from pCVD442

This study

 pHK1009

pDK46 with an insertion of Km^R cassette from pKD4

This study

 pHK1014

pKD46 with an insertion of Gm^R cassette from pJN105

[27]

 pSY005

pHK251 containing ompR

This study