Reduced virulence in tigecycline-resistant Klebsiella pneumoniae caused by overexpression of ompR and down-regulation of ompK35

Table 2 Primers used in this study

Primers	Sequence (5′ → 3′)	Reference
Primers for cloning
ompR-F/BamHI^a	TGAGAGGATCCATGCAAGAGAATTATAAGAT	This study
ompR-R/BamHI^a	CTACAGGATCCTCATGCCTTAGAACCGTCCG	This study
ompR-F	GGTGATCAGCGGCGTTTTCAT	This study
ompR-R	AGTGTGCGAGCAAAGGAGCT	This study
Primers for deletion		This study
Del-ompR-F^b	ACGCACTGACTATTGCAGTGAACCTTTGGGAGTACAAACATGTAGGCTGGAGCTGCTTCG	This study
Del-ompR-R^b	GTGCGAGCAAAGGAGCTGCGCGGCGAAAAGCGCACGCGTTCATATGAATATCCTCCTTAG	This study
Primers for verification		This study
Checkpri-ompR-F	CAGTTTTTCATATCCCTCGCG	This study
Checkpri-ompR-R	CTGCAGTTTGTCGGTCATCA	This study
pKD46-repA101-F	CCCCACGATTGAAAACCCTACAAGG	This study
pKD46-repA101-R	GGAAAATCAACGTATCAGTCGGGCG	This study
Primers for qRT-PCR		This study
Q-ompR-F	ACCGAGCAGGGCTTCCA	This study
Q-ompR-R	CAGCATCAGATCCAGCACCAT	This study
Q-ompK35-F	TACGGCCAGTGGGAATACAAC	This study
Q-ompK35-R	CGTATTCGCCCGCTTTCA	This study

^aBold sequences refer to restriction enzyme cutting sites
^bUnderlined sequence refer to complementary to the plasmid sequence for overlapping PCR

ISSN: 1423-0127