Fig. 2
Lytic reactivation of EBV alters m6A Modification of TYK2 and DTX4 mRNA
A, B. Coverage plot of MeRIP (color) and input (black) reads in TYK2 and DTX4 transcripts in Akata and LCL cells during latency and reactivation, as determined by MeRIP-seq. Average normalized plot from the replicate experiments were represented. The gray shades represent the standard deviation of the duplicate samples. The dotted lines and black arrows represent regions on TYK2 and DTX4 gene that were hypermethylated during reactivation. Red arrow represents the regions that were hypermethylated during reactivation and are having the RRACH motif. C. MeRIP-rtPCR analysis of relative m6A level of TYK2 and DTX4 in latent and reactivated LCL and Akata cells. Fold enrichment was determined by calculating the fold change of IP to input Ct values. IgG precipitated RNA enrichment was used as the control. D. RNA expression of TYK2 and DTX4 during latency and reactivation and normalized based on GAPDH. E. Alteration of protein expression of TYK2 and DTX4 during reactivation in LCL and Akata cells. Reactivation was confirmed by expression of BZLF1 and gp350. Experiments were independently repeated three times, and results are presented as mean+/-s.d. from the three experiments. “***” represents p-value < 0.001; “**” represents p-value < 0.01; “*“represents p-value < 0.05 and “ns” represents no significance