Fig. 3
m6A methylation alters metabolism of TYK2 and DTX4 mRNA
A, B. The rtPCR quantification of relative DTX4 and TYK2 RNA in nuclear and cytoplasmic fractions in latent and reactivated LCL and Akata cells. C, D. Measurement of TYK2 and DTX4 mRNA in latent and reactivated LCL and Akata cells. At 12, 24 and 36 h post reactivation, cell culture media was replaced with media containing Actinomycin D (ActD). RNA was harvested from cells at 36 h post-reactivation subjected to rtPCR to determine remaining relative TYK2 and DTX4 mRNA levels. E, F. Relative absorbance values of fractions 1–16 retrieved from ultracentrifugation of latent and reactivated LCL and Akata cell extracts over 15–50% sucrose gradients (top panel). Percent of mRNA in each set of fractions. Messenger Ribonucleoprotein (mRNP), 80S Ribosome fraction and polysome and polyribosome fraction (middle and bottom panel). Experiments were independently repeated three times, and results are presented as mean ± s.d. from the three experiments. “***” represents p-value < 0.001; “**” represents p-value < 0.01; “*“represents p-value < 0.05 and “ns” represents no significance