Fig. 5
Effect of ALKBH5 on the Interferon signaling
(A) Protein expression of ALKBH5 in latent and reactivated LCL and Akata cells. (B) Effect of knockdown of ALKBH5 on TYK2 and DTX4 protein levels in LCL and Akata cells. C, D. MeRIP-rtPCR analysis of relative m6A level of TYK2 and DTX4 in knock down ALKBH5 condition of latent and reactivated LCL and Akata cells. E. Effect of knockdown of ALKBH5 on viral copy numbers per milliliter during latency and reactivation. F. Alteration of levels of IRF9 and STAT protein expression in nuclear and cytosolic fraction of ALKBH5 knock down cells. G. Microscopic analysis of the expression of IRF9 in LCL and AKATA cells during ALKBH5 knock down conditions. Experiments were independently repeated three times, and results are presented as mean+/-s.d. from the three experiments. “***” represents p-value < 0.001; “**” represents p-value < 0.01; “*“represents p-value < 0.05 and “ns” represents no significance