Fig. 2

Examination of the C-TDP-43 disaggregation and beneficial effects of PROTACs 1–4. A Representative images of eGFP-C-TDP-43-expressing Neuro-2a cells with or without PROTAC 1–4 (5 μM). Scale bar = 10 μm. B Filter trap assay of eGFP-C-TDP-43 expressed Neuro-2a cells in the presence and absence of PROTACs 1–4 (5 μM). The cell lysate was either loaded on cellulose acetate (CA) or nitrocellulose (NC) membrane probed with TDP-43 (C-terminal) antibody and β-actin antibody (loading control), respectively. C Quantification of blots in panel B. D AlamarBlue reduction assay of eGFP-C-TDP-43 expressed Neuro-2a cells treated with PROTACs 1–4 (5 μM). E Western blot of eGFP-C-TDP-43 transfected Neuro-2a cells treated with various concentrations of PROTAC 2. The RIPA-insoluble fraction and RIPA-soluble fraction of Neuro-2a lysate were further probed with GFP and GAPDH antibody, respectively. All the statistic results were quantified by ImageJ and shown as mean ± SD (n ≥ 3). Data were analyzed by one-way ANOVA with Dunnett post-hoc test (*P < 0.05, **P < 0.01, ***P < 0.001)