Fig. 3

Schwann cell CGRP receptor activation mediates periorbital mechanical allodynia (PMA) evoked by ethanol (EtOH) and acetaldehyde (ACD). a, b Time-dependent PMA evoked by EtOH (15%, 4 ml/kg, i.g.), ACD (0.1 mg/kg, i.p.), or vehicle (veh, 0.9% NaCl) in C57BL/6J mice treated (30 min before) with olcegepant (1 nmol, periorbital, p.orb.) or veh. c, d PMA evoked by EtOH (15%, 4 ml/kg, i.g.), acetaldehyde (ACD, 0.1 mg/kg, i.p.), or veh in Plp1-Cre^ERT+;Ramp1^fl/fl and Plp1-Cre^ERT−;Ramp1^fl/fl (control) mice. PMA evoked by EtOH (15%, 4 ml/kg, i.g.), acetaldehyde (ACD, 0.1 mg/kg, i.p.), or vehicle (veh, 0.9% NaCl) in C57BL/6J mice treated (30 min before) with e, f SQ-22536 (25 nmol, p.orb.), g, h H89 (45 nmol, p.orb.), i, j L-NAME (1 mmol, p.orb.), k, l cPTIO (200 nmol, p.orb.), or veh. (n = 8 mice). *P < 0.05 vs. veh; ^#P < 0.05 vs. veh olcegepant, SQ-22536, H89, L-NAME, cPTIO, control -EtOH, two-way ANOVA with Bonferroni post-hoc correction