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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Identification of three capsule depolymerases in a bacteriophage infecting Klebsiella pneumoniae capsular types K7, K20, and K27 and therapeutic application

Fig. 6

Alcian blue staining of exopolysaccharide treated with K7dep, K20dep, or K27dep. Exopolysaccharide (EPS; 8 μg) extracted from Klebsiella K7 (K7ref), K20 (A13), K27 (K27ref), and K1-type (NTUH-K2044) strains was treated with or without 3 μg of K7dep, K20dep, or K27dep in 15 μl of reaction volume at 37 °C for 1, 4, or 24 h and then visualized by Alcian blue stained SDS-PAGE gels. Ctrl (control) indicates no enzyme added. A different capsular-type strain, NTUH-K2044 (K1-type), abbreviated as 2044, was used as a control. BLUeye prestained protein ladder (GeneDireX) was used to indicate molecular weights from ~ 11 to ~ 245 kDa on the gels

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Journal of Biomedical Science

ISSN: 1423-0127