Fig. 2

The demonstration of BsAb-based culturing platform. Human PBMCs were isolated from the blood of healthy donors and co-cultured with four types BsAb or traditional mouse anti-CD3 antibody (OKT3), respectively. After 14 day culturing, the (A) T cell population was analyzed by using flow cytometry with anti-CD3 antibody, anti-CD4 antibody, or anti-CD8 antibody staining. B The surface OKT3 or BsAb were detected by anti-mouse antibody or anti-His antibody respectively. The cell expansion rate of (C) total cells or (D) CD3⁺ CD8⁺ T cells cultured by OKT3 or BsAbs were calculated on consecutive days. Key: bar, SD; *p < 0.05; **p < 0.01; ***p < 0.001