Fig. 4
CREBH promotes intestinal epithelial regeneration and wound healing via mediation of IGF signalling. A mRNA expression of IGF1R in the colon tissues of wild type (WT, C57BL/6J) and CREBH-KO mice (n = 5–10/groups) treated with DSS or vehicle (Veh, H2O) in drinking water for 14 days. B qRT-PCR analysis of mRNA expression of IGF1R in IPEC-J2 cells transfected with mock (empty vector) or an active CREBH (N-terminal CREBH, constitutively active) plasmid for 48 h. C Immunoblotting analysis of plasma IGF-1 and loading control Albumin in the plasmas of WT and CREBH-KO mice (n = 5–6/group). D mRNA expression of IGF1R in the colon tissues of WT and CREBH-KO mice (n = 5–10/groups) pre-treatment with A. muciniphila (AKK) or Veh (PBS) followed by DSS or Veh treatment as described in the "Methods". E IEC wound healing assay: Caco2 cells transfected with mock (empty vector) or an CREBH expressing plasmid were grown to a confluent monolayer before being wounded by scratching and monitored for rate of wound healing by microscopy imaging; rate of wound healing is presented by the ratio (%) of wounded areas at each indicated time point to its initial wounded area at time 0 as described in the Additional file 1: Supplemental Methods. F Representative images of the same wound at 0 and 30 h are presented. Results represent the means ± SD. The two-tailed Student’s t-test was used for statistical analyses of two-group comparisons. *P < 0.05 and **P < 0.01 versus controls