Fig. 4
UVA-induced PARP-1 activation can reduce DNA damage and subsequent cell death. ARPE-19 cells were pre-treated with NAC (5 mM) (A) or olaparib (10 µM) (B–D) 30 min prior to UVA (12.6 J/cm2) irradiation. A, B After UVA exposure at the indicated time points cell lysates were collected for immunoblotting. C Cell viability was determined by Annexin V-FITC/PI at 18 h post-UVA irradiation. D Mitochondrial ROS level was detected by MitoSOX staining at 1 h after UVA. Data were the mean ± S.E.M. of 3 independent experiments. *p < 0.05, indicating the significant effects of UVA; #p < 0.05, indicating the significant effects of olaparib to enhance cell death and increase ROS production