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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: SCEL regulates switches between pro-survival and apoptosis of the TNF-α/TNFR1/NF-κB/c-FLIP axis to control lung colonization of triple negative breast cancer

Fig. 1

iTRAQ-based LC MS/MS approach identifies a unique membrane-related protein profiles in the metastatic TNBC cells derived from the metastatic lung nodules. A A schematic of identification of metastasis-related membrane proteins using iTRAQ-coupled LC MS/MS. B Subcellular protein fractionation of 231-PT, IV2 and LC cells. C MS-generated proteomic data were quantified and identified by proteome discoverer software and Mascot search engine, respectively. A total of 7538 proteins from three cellular compartments of 231-PT, IV2, LC cells was successfully identified and quantified. D Average of MS intensity ratio (log2) of IV2 versus 231-PT and LC versus 231-PT. E Venn diagram analysis of the proteins with 1.5-fold increase from three compartments. The metastasis-related proteins that were upregulated at least 1.5-fold in the membrane fraction of IV2 cells (shown as the blue circle) and LC cells (shown as the orange circle) as compared to that of the 231-PT cells were identified. F Venn diagram analysis of IV2-specific and LC-specific membrane-associated protein

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Journal of Biomedical Science

ISSN: 1423-0127