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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: SCEL regulates switches between pro-survival and apoptosis of the TNF-α/TNFR1/NF-κB/c-FLIP axis to control lung colonization of triple negative breast cancer

Fig. 3

SCEL is a membrane associated protein that are exclusively expressed in the LC sublines derived from the metastatic lung nodules. A The protein expression of SCEL was compared in the multiple clones of MDA-MB-231-PT cells, and its sublines IV2, and LC cells isolated from the TNBC xenograft mouse model. Lower panel. The protein expression of SCEL was compared in a series of BC cell lines including luminal, HER2, and TNBC subtypes. B Immunohistochemistry staining of SCEL expression in LC cells-derived lung metastatic nodules in the tail vein injection mouse model. C Analysis of SCEL localization in LC cells-derived lung nodules using Image J software. Three nodules per mouse and a total of five mice were included in the analysis. Membrane staining of SCEL indicated by black arrows. D Subcellular localization analysis of SCEL in LC cells by protein fractionation. E The confocal immunofluorescence images of the HA-SCEL-transfected Hs578T cells. Membrane localization of SCEL indicated by white arrows. Scale bar, 25 μm. All cell-based experiments were performed in triplicates and repeated at least three times. The representative results are shown. *P < 0.01

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Journal of Biomedical Science

ISSN: 1423-0127