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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: SCEL regulates switches between pro-survival and apoptosis of the TNF-α/TNFR1/NF-κB/c-FLIP axis to control lung colonization of triple negative breast cancer

Fig. 4

Knockdown of SCEL expression significantly reduces lung colonization potential of lung-tropic LC cells and prevents metastasis-caused death in a mouse model. A Western blot analysis of SCEL stable knockdown LC cells. B Transwell migration and invasion analysis of the control LC cells and two SCEL stable knockdown cells. C Analysis of anchorage-independent growth of SCEL-downregulated LC cell and the control LC cells using 3D-colony-forming assay. *P < 0.05. D Upper images showed the mouse lungs dissected from SCID mice intravenously injected with the control LC cells (n = 4) or SCEL-downregulated LC cells (n = 4). Bottom images showed HE staining of the mouse lung sections from the control group and the SCEL knockdown group. E Quantitative analysis of lung metastasis of mice intravenously injected with the control LC cells or SCEL-downregulated LC cells. *P < 0.001. F The Kaplan-Meir survival analysis of mice intravenously injected with the control LC cells or SCEL-downregulated LC cells. For the cell-based experiments, each experiment was performed in triplicates and was repeated at least 3 times

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Journal of Biomedical Science

ISSN: 1423-0127