Fig. 6

Loss of SCEL switches TNF-α-mediated cell survival to apoptosis in the lung-tropic metastatic TNBC cells. A Western blotting analysis of TNF-α-induced NF-κB-mediated pro-survival/apoptotic and proliferation signals in SCEL stable knockdown LC and the control LC cells. B The representative immunofluorescence (IF) images of cleaved caspase 3 in TNF-α-treated control LC and SCEL stable knockdown LC cells. Scale bar, 20 μm. C Quantitative result of the IF staining of TNF-α-induced cleaved caspase 3 in TNF-α-treated control LC and SCEL stable knockdown LC cells. *P < 0.01. D Evaluation of the capacity for anchorage-independent growth in control LC and SCEL-downregulated LC cells following TNF-α stimulation. *P < 0.05. E MTS cell proliferation analysis of the control LC and SCEL-downregulated LC cells in the presence or absence of TNF-α. *P < 0.05. F Western blotting analysis of TNF-α-induced NF-κB-mediated pro-survival/apoptotic and proliferation signals in SCEL-overexpressing 231 and the control 231 cells. G The representative immunofluorescence (IF) images of cleaved caspase 3 in TNF-α-treated SCEL-overexpressing 231 and the control 231 cells. Scale bar, 20 μm. H Quantitative result of the IF staining of TNF-α-induced cleaved caspase 3 in TNF-α-treated SCEL-overexpressing 231 and the control 231 cells. *P < 0.01. I Evaluation of the capacity for anchorage-independent growth in SCEL-overexpressing 231 cells and the control 231 following TNF-α stimulation. *P < 0.05. J MTS cell proliferation analysis of SCEL-overexpressing 231 and the control 231 cells in the presence or absence of TNF-α. *P < 0.05. Each experiment was performed at least three times