Fig. 7

SCEL interacts with TNFR1 and plays a crucial role in maintaining its protein stability. A Western blotting analysis of protein expression of TNFR1 complex II in the SCEL stable knockdown LC cells and the control LC cells. B Protein degradation analysis of TNFR1 in the control LC cells and the SCEL-downregulated cells following 20 μg/mL cycloheximide (CHX) treatment. C Quantification of protein degradation kinetics of TNFR1 in the control LC and the SCEL-downregulated cells following 20 μg/mL cycloheximide (CHX) treatment. *P < 0.05. D Western blotting analysis of protein expression of TNFR1 complex II in SCEL-overexpressing 231 and the control 231 cells. E Protein degradation analysis of TNFR1 in in SCEL-overexpressing 231 and the control 231 cells in response to 20 μg/ml cycloheximide (CHX) treatment (top). Quantification of protein degradation of TNFR1 (bottom). *P < 0.01. F Analysis of protein interaction between SCEL and TNFR1 using reciprocal co-immunoprecipitation (co-IP) G Analysis of protein interaction between SCEL and TNFR1 using reciprocal co-immunoprecipitation (co-IP). H Examination of interaction between SCEL and TNFR1 through GST pull-down assay. Each experiment was repeated at least 3 times