Fig. 5
From: Scaffold-based 3D cell culture models in cancer research
illustration of the microfluidic chip used in 3D co-culture of human colorectal cancer cells (HT-29) and normal colorectal fibroblasts (CCD-18Co) in a collagen matrix. The chip comprised 4 units, each featuring 7 channels for cell loading or media fill. Cancer and fibroblast cells were loaded into channels 4 and 2 in the co-culture, while channels 1 and 3 were designated for media fill. A cell loading channel’s detailed structure and dimensions are illustrated at the bottom left. Figure adapted from [145]