Fig. 3
From: Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids as an evolving tool
In vitro tumor spheroids as suitable tools to study cell adhesion molecules. a Given the high heterogeneity of GB tumors, P-selectin expression was studied in spheroids of mesenchymal (iAGR53 cell line), proneural (PNp53 cell line) and classical (EGFRviii-shP16 cell line) murine GB cell subtypes, when co-cultured in 3D with murine microglia and in their respective 2D controls. These distinct GB cell subtypes expressed high levels of P-selectin compared to 2D cultures and even higher levels when co-cultured with murine microglia. Adapted under the terms of CC 4.0 license from [121]. Copyright 2021, the authors. bi Confocal microscopy images of soluble E-selectin (orange) binding to BT20 (blue, B), MCF7 (green, C) or MCF10A (red, C) cells in whole co-culture spheroids (superimposed, A) on polydimethylsiloxane (PDMS) bii Flow cytometry histogram for soluble E-selectin binding in monolayer, spheroid, monolayer co-culture and spheroid co-culture with respect to isotype control for BT20 (A), MCF7 (B) and MCF10A (C) cells. Adapted with permission from [122]. Copyright 2012, Elsevier