Fig. 5

SNHG6 KD promotes erythrocyte differentiation and prevents megakaryocyte differentiation in K562 cells. a Cell proliferation was significantly reduced by both knockdown (KD) and knockout, while overexpression in KD samples restored proliferation rates. b GPA levels were normalized to those in untreated cells in each sample to quantify the degree of erythrocyte differentiation. SNHG6 KD promoted erythrocyte differentiation. Error bars represent SD (n = 3 biological replicates). *p < 0.05, **p < 0.01 vs. sgRNA control. c CD41/CD61 levels were normalized to basal levels in each sample to quantify the degree of megakaryocyte differentiation. SNHG6 KD significantly reduced megakaryocyte differentiation after 72 h. Error bars represent SD (n = 3 biological replicates). **p < 0.01 vs. sgRNA control. d–f SNHG6 KD led to reduced tumor volume (e) and weight (f) in a xenograft mouse model. d Representative tumors. Error bars represent SEM. *p < 0.05 vs. sgRNA control by one-way ANOVA (n ≥ 11 from two independent experiments). g Proposed model for the mechanism of function of SNHG6