Fig. 10

Self-cleaving Cas9 leads to substantial increases in knock-in fidelity with the long homology arm strategy. Each strategy was plotted into the dot graph for unselected GFP+ and selected GFP+BFP− results. The graph is divided into four quadrants at the 50% scale on each axis. Grouped samples are generated from the same strategy which consist of Cas9, Cas9es, Cas9-Gem, and Cas9es-Gem variants. Colour code was appointed to indicate high efficiency and fidelity (green), high efficiency with low fidelity (yellow), low efficiency with high fidelity (red), and low efficiency and fidelity outcomes (blue)