Fig. 11

Inhibition of NHEJ or HR leads to small but significant increases in faithful integration of short HA donors. HEK293T cells were transfected with Cas9 and sgRNA plasmids along with the donor plasmid for either the unmatched ends, matched ends, long HA, or short HA strategy. A Cells were analysed for GFP+ only expression by flow cytometry 2 days after transfections. Error bars indicate standard error from three biological replicates. B Transfected cells were grown under puromycin selection for 3 weeks before final flow cytometry analysis. Error bars indicates standard error from ≥ two biological replicates. *p < 0.05; **p < 0.01 in post-hoc Tukey (Additional file 1: Tables S2 and S3)