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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Improving CRISPR–Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration

Fig. 6

Distinct integration profiles of stable puromycin selected cells from overhang mediated KI strategies. Transfected cells were grown under puromycin selection for 3 weeks before final flow cytometry analysis. Stable GFP+BFP− cells were quantified using flow cytometry and compared to non-Geminin results. A Strategies utilizing 5′ overhang DNA DSBs with various lengths of overhangs; B Strategies utilizing 5′ overhang DNA DSBs with various lengths of overhangs. Error bars indicates standard error from ≥ three biological replicates. *p < 0.05

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Journal of Biomedical Science

ISSN: 1423-0127