Fig. 7

Geminin-restricted Cas9es expression increases effective transgene integration in overhang DSB-mediated strategies. HEK293T cells were transfected with plasmids expressing either Cas9, Cas9es, Cas9-geminin or Cas9es-geminin, alongside the sgAAVS1-A1 plasmid and the donor plasmid for either the matched ends, long HA, or short HA strategy. Cells were analysed for GFP expression by flow cytometry 2 days after transfections. A Strategies utilizing blunt end DNA DSBs; B strategies utilizing overhang DNA DSBs. Error bars indicate standard error from three biological replicates. *p < 0.005