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Fig. 8 | Journal of Biomedical Science

Fig. 8

From: Improving CRISPR–Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration

Fig. 8

Geminin-restricted Cas9es expression increases faithful transgene integration in HA strategies. Transfected cells were grown under puromycin selection for 3 weeks before final flow cytometry analysis. Stable GFP+BFP− cells were quantified using flow cytometry and compared to non-Geminin results. A Strategies utilizing blunt end DNA DSBs; B strategies utilizing overhang DNA DSBs. Error bars indicates standard error from ≥ three biological replicates. *p < 0.05

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Journal of Biomedical Science

ISSN: 1423-0127