Table 3 Strategies that produced a significant change in initial transgene integration efficiency in K562 compared to HEK293T
Strategy | Cas9 variant | Log2 fold differences |
|---|---|---|
5′ overhang-pair 1 | Cas9es | 2.995068471 |
Long HA | Cas9es | 2.434625651 |
Short HA | Cas9es | 2.2101824 |
Matched ends | Cas9es | 2.019033767 |
3′ overhang-pair 1 | Cas9es | 1.261492241 |
Short HA | Cas9-Gem | 0.753938673 |
Long HA | Cas9-Gem | 0.62552389 |
3′ overhang-pair 1 | Cas9-Gem | − 0.889219569 |
3′ overhang-pair 1 | Cas9es-Gem | − 1.073854847 |
5′ overhang-pair 1 | Cas9 | − 1.330448081 |
Matched ends | Cas9-Gem | − 1.469485283 |
Matched ends | Cas9 | − 2.423440966 |
5′ overhang-pair 1 | Cas9es | − 3.482663925 |
5′ overhang-pair 1 | Cas9-Gem | − 4.743391863 |