Fig. 4

HBc dephosphorylation is neither necessary nor sufficient for viral DNA synthesis. A Eleven RNase H domain mutants were designed to contain substitutions from a hydrophobic to an acidic residue. B Viral DNA synthesis of these RNase H mutants was analyzed by Southern blot analysis. Red asterisks highlight mutants with dissociated activities of viral DNA synthesis in B) and HBc dephosphorylation in C). C Phos-tag gel analysis detected the presence or absence of dephosphorylated HBc protein. D A summary table of five RNase H domain mutants (red asterisk) which exhibited dissociation between HBc dephosphorylation and viral DNA synthesis