Fig. 2

TRAIL-R inhibits proximal TCR signaling without activation of apoptosis signaling. a Immunoblotting of caspase-8, caspase-3, and proximal TCR signaling molecules (30 min), and (b) Flow cytometry of Annexin V⁺ apoptotic cells (24 h) from murine splenic CD4.⁺ T cells (WT) or Jurkat cells stimulated with anti-CD3 (3 µg/mL) and anti-CD28 (2 µg/mL) Abs with or without TRAIL (10 µg/mL) in the presence or absence of pan-caspase inhibitor, Z-VAD-FMK (10 µg/mL). Data in (b) represent analyses from three independent experiments. Statistical significance was determined using the Mann–Whitney U test; NS, no significance; *** p < 0.001