Fig. 4

SHP-1 is associated with TRAIL-R in T cells. a, b Immunoprecipitation analyses of tyrosine phosphatases SHP-1, SHP-2, Cbl, and Csk with TRAIL-R in murine splenic CD4⁺ T cells in the presence of TRAIL (10 µg/mL) at indicated time point. Quantification of phosphorylated protein levels are shown at the bottom of the panel. Data represent analyses from at least three independent experiments; statistical significance determined by two-tailed Student’s t test; NS, no significance; * p < 0.05; *** p < 0.001. c, d Co-immunoprecipitation of TRAIL-R with SHP-1 or SHP-2 from murine splenic CD4⁺ T cells stimulated with anti-CD3 (3 µg/mL) and anti-CD28 (2 µg/mL) Abs in the presence or absence of TRAIL (10 µg/mL) for 30 min. Quantification of phosphorylated protein levels are shown at the bottom of the panel. Data represent analyses from at least three independent experiments; statistical significance determined by two-tailed Student’s t test; NS, no significance; *** p < 0.001. e Immunoprecipitation analyses of TRAIL-R with SHP-1 or SHP-2 in TRAIL-R^WT-FLAG or TRAIL-R^△DD-FLAG T cells stimulated with anti-CD3 (3 µg/mL) and anti-CD28 (2 µg/mL) Abs in the presence or absence of TRAIL (10 µg/mL) for 30 min