Fig. 4
In vivo efficacy of Tha-PA90 in a mouse model of A. baumannii systemic infection. A BALB/c mice, treated with CPM, were intraperitoneally injected with 1 × 10.8 CFU of A. baumannii ATCC 19606. One hour post infection, the animals received an intraperitoneal injection of mock (1 × PBS), PA90, or Tha-PA90 (600 μg/mice). Survival rates were monitored for 52-h. B After 10 h post-infection, mice were euthanized, and organs were isolated to enumerate bacterial load by plating serial dilutions of homogenized organs. C Serum levels of IL-6 or TNF-α in mock or Tha-PA90-treated mice were analyzed with ELISA 10-h post-injection. Significance is indicated as ***p < 0.0006, *p < 0.0468. D Expression levels of IL-6 or TNF- α from liver tissues were evaluated by measuring mRNA relative to GAPDH qPCR. All experiments were performed in triplicate. Significance is indicated as ***p < 0.0006, *p < 0.0463