The effects of the bacterial interaction with visible-light responsive titania photocatalyst on the bactericidal performance
© Cheng et al; licensee BioMed Central Ltd. 2009
Received: 24 October 2008
Accepted: 15 January 2009
Published: 15 January 2009
Bactericidal activity of traditional titanium dioxide (TiO2) photocatalyst is effective only upon irradiation by ultraviolet light, which restricts the potential applications of TiO2 for use in our living environments. Recently carbon-containing TiO2 was found to be photoactive at visible-light illumination that affords the potential to overcome this problem; although, the bactericidal activity of these photocatalysts is relatively lower than conventional disinfectants. Evidenced from scanning electron microscopy and confocal Raman spectral mapping analysis, we found the interaction with bacteria was significantly enhanced in these anatase/rutile mixed-phase carbon-containing TiO2. Bacteria-killing experiments indicate that a significantly higher proportion of all tested pathogens including Staphylococcus aureus, Shigella flexneri and Acinetobacter baumannii, were eliminated by the new nanoparticle with higher bacterial interaction property. These findings suggest the created materials with high bacterial interaction ability might be a useful strategy to improve the antimicrobial activity of visible-light-activated TiO2.
The widespread use of antibiotics and the emergence of more resistant and virulent strains of microorganisms [1–3] have caused an urgent need to develop alternative sterilization technologies. Using the superb photocatalytic effect of titanium dioxide (TiO2) is a conceptually feasible technology for this material is easy and inexpensive to produce in industrial scale. Photocatalytic TiO2 substrates have been shown to eliminate organic compounds and to function as disinfectants . Upon ultraviolet (UV) light excitation, the photon energy excites valence band electron and generates pairs of electrons and holes (electron-vacancy in valence band) that diffuse and are trapped on or near the TiO2 surface. These excited electrons and holes have strong reducing and oxidizing activity and react with atmospheric water and oxygen to yield reactive species such as hydroxyl radicals (.OH) and superoxide anions (O2-) . These radicals, .OH and O2- are extremely reactive upon contact with organic compounds. Complete oxidation of organic compounds and bacterial cells to carbon dioxide could be achieved [6, 7]. Reactive oxygen species (ROS), such as .OH, O2-, and hydrogen peroxide (H2O2) generated on the light irradiated TiO2 surfaces, were shown to operate in concert to attack polyunsaturated phospholipids in bacteria . Traditional TiO2 photocatalyst, however, is effective only upon irradiation of UV-light at levels that would also induce serious damage to human cells. This greatly restricts the potential applications of TiO2 substrates for use in our living environments. Recently, nitrogen or metal ion-doped anatase based TiO2 photocatalysts have been identified to be active upon visible-light illumination [8, 9], offering the possibility to overcome this problem.
It is believed that nanometer-sized anatase phase particles have large surface area are efficient for the decomposition of pollutants in air and in water . Furthermore, it is also found that the presence of anatase and rutile phases is important in some of the photocatalytic reactions where oxygen is used as electron acceptor . Transmission electron microscopy studies also revealed that commercial TiO2 powder Degussa (P-25) consisting both anatase and rutile phases . However, in these studies, the photocatalytic activities were induced under UV irradiation (wavelength < 380 nm). Previously, we have produced carbon-containing TiO2 in two different calcination temperatures (150°C and 200°C) resulted in two different nano-crystals (labeled as C150 and C200, respectively) with photocatalytic activity in the visible-light range . These materials seem to be more convenient to apply in our living environment than the commercial UV responsive photocatalysts. The antibacterial activity of visible-light responsive photocatalysts has been reported by several groups [13–15]. Since photocatalyst-based anti-microbial technologies are still under development, the antibacterial activity of these materials does not match to that of conventional chemical disinfectants [13, 16]. To improve the antibacterial activity, previous studies were mainly focused on the photocatalysis properties [17, 18], while the photocatalyst-bacterial interactions were rarely discussed.
In this present study, scaning electron microscopy and confocal Raman spectrscopy were used to study different photocatalysts interact with pathogens. The photocatalyst-bacterial interaction properties were then compared to the bactericidal activity of respective photocatalysts. To further investigate whether the antibacterial effect can be generally applied to human pathogens, we tested several human pathogens including Staphylococcus aureus, Shigella flexneri and Acinetobacter baumannii. Among these bacteria, S. flexneri is a food-borne pathogen, which is usually found in contaminated water, plants, and sewage [19–22], and frequently leads to outbreaks in regions with poor sanitary conditions [21, 23]. S. aureus is a exotoxin producing pathogen which can cause diseases such as food-borne diseases, soft tissue infections, and toxic shock syndrome in humans. The emergence and rapid spread of multidrug-resistant A. baumannii isolates causing nosocomial infections are of great concern worldwide . The antimicrobial performance of the visible-light responsive titania catalysts against these bacteria will be compared.
Materials and methods
Preparation of TiO2, C150 and C200 nanoparticles
Carbon-containing mixed phase nano-structured TiO2 powders were prepared using a modified sol-gel method. The produced powders were subjected to calcination at 150°C and 200°C, and named as C150 and C200, respectively. Details in preparation of C150 and C200, structural properties, the sizes of primary particles, light absorption, etc. have been reported elsewhere . In our previous study , we found the C200 has a unique anatase/rutile mixed crystalline phases that exhibits strong visible-light absorption and photocatalytic effects. The photocatalytic studies have been reported previously [12, 25, 26]. In these TiO2, carbons exist in an amorphous form as seen in the Raman spectra, and the carbon contents were estimated using x-ray photoelectron spectroscopy to be approximately ~30 atomic % on the surface (data not shown). One commercially available TiO2 nanopowder (UV100, Sachtleben, Germany), that can exert the photocatalytic property only when illuminated by UV light, was used for comparison. Since C150 and C200 samples often aggregate into larger cluster due to surface charges, Van der Waals interactions, we dispersed the aggregates using sonication (Transsonic digital TP680DH, Ultrasonic cleaning Co. Singapore, Singapore) before the bacteria-killing or bacteria-photocatalyst interaction experiments.
Confocal Raman spectral mapping
Confocal Raman mapping was carried out with a confocal Raman spectrometer using 488 nm excitation wavelength (α-SNOM, Witec, Germany). The confocal Raman mapping has a spatial resolution of ~250 nm; typical scan were performed in an area of 10 × 10 μm2 area and in air. The mapping consisted of 0.2 μm in each step in both the x and y directions, with specific Raman signals of the interested sample components are plotted to form a 2-D map to reveal the structural distribution of the interested structures. The bacteria-nanoparticle images were taken after 20 μl of nanoparticles (10 mg/ml) and bacteria (1 × 106 CFU/ml) suspensions in H2O were spread on cover glasses and dry. Laser power were kept low (less than 1 mW) to avoid damaging the test samples, both the TiO2 and the bacteria.
Scanning electron microscopic imaging
Scanning electron microscopic (SEM) analysis was performed as previously described [27–30]. The images were obtained using a JEM-3010 scanning electron microscope (JEOL, Japan) equipped with energy dispersive x-ray spectrometer (EDS) for the chemical elemental analysis to observe the surface morphology of the tested TiO2 nanoparticles. To observe the interaction of microbes and TiO2 samples, bacteria and TiO2 powders were mixed and subjected to photocatalytic reaction as described in next sections. After the reaction, the samples were transferred to cover-glasses and fixed by 2.5% glutaraldehyde in 0.1 M phosphate buffer, then 1% osmium tetroxide in 0.1 M phosphate buffer, pH 7.3, and then subjected to a series of alcohol dehydration, critical point drying procedures, and gold coating  and observed under a scanning electron microscope at 15 kV (Hitachi S-4700, Hitachi, Japan). At least three different areas were randomly selected for photography at each magnification; representative data are shown.
Bacterial strains and culture
Basic bacterial cultural methods were performed as previously described [13, 31]. Clinical isolated S. flexneri was collected from a shigellosis outbreak in central Taiwan in 1996 . A. baumannii, pan-drug resistant A. baumannii and S. aureus were clinical isolates from Buddhist Tzu-Chi General Hospital in Hualien, Taiwan. All isolates were initially differentiated into Gram positive and Gram-negative strains by a standard staining procedure. The bacteria were cultured in tryptic soy broth supplemented with 0.5% yeast extract (TSBY) and LB at 37°C for 16 hr, and then identified by biochemical methods according to routine clinical laboratory procedures . S. flexneri, A. baumannii and pan-drug resistant A. baumannii were maintained and grown in LB medium or LB agar at 37°C. Bacterium S. aureus was grown in TSBY broth or TSBY broth agar (MDBio, Inc. Taipei, Taiwan) at 37°C. All bacteria isolates were stored in 50% glycerol (V/V) in culture medium at -80°C before use. To reactivate bacteria from frozen stocks, 25 μl bacterial stock solution was transferred to a test tube containing 5 ml of freshly prepared culture medium and then incubated at 37°C under agitation overnight (16–18 hr).
Bactericidal effects of the TiO2 nanoparticles
In this study, bacterial concentrations were either determined by the standard plating method or inferred from optical density readings at 600 nm (OD600). For each bacterium, a factor for converting the OD600values of the bacterial culture to concentration (CFU/ml) was calculated as the followings. A fresh bacterial culture was diluted by factors of 10-1 to 10-7, and OD600 of these dilutions was measured. Bacterial concentrations of these dilutions were determined using standard plating method. The OD600 values were plotted against the bacterial concentrations' log values, and the conversion factors for particular bacteria were calculated. The conversion factor for S. aureus, for example, was calculated to be 1 × 108 CFU/ml per OD600 by this method.
In order to determine the bactericidal effects of the TiO2 nanoparticles, 200 μl of bacterial overnight culture was transferred into 5 ml of culture medium and incubated at 37°C until an OD600 of 0.3 to 0.6 (log phase) was reached. The bacterial concentrations were calculated using the conversion factor for the bacteria, and the cultures were diluted to 5 × 105 CFU/ml with culture medium. Fifty micro liters of the bacterial culture (2.5 × 104 CFU) were mixed with the TiO2 nanoparticles (200 μg/ml in 150 μl normal saline) using a plastic yellow tip and placed onto a 24-well cell culture dish. The cell culture dish was then placed under an incandescent lamp (Classictone incandescent lamp, 60W, Philips, Taiwan) for photocatalytic reaction, and a light meter (model LX-102, Lutron Electronic Enterprises, Taiwan) was used to record the illumination density. In the dose-dependence experiments, illuminations were carried out for 5 min at a distance of 5 and 15 cm from the lamp, corresponding to the illumination density of 3 × 104, and 5 × 102 lux (lumen/m2)(90 and 10 mW/cm2), respectively. In the kinetic analysis experiments, illuminations were carried out for 1, 5, 10, 20, and 40 min at a distance of 5 cm, corresponding to an illumination density of 3 × 104 lux (90 mW/cm2). After illumination, the bacterial solutions were recovered from the 24-well cell culture dishes, and an aliquot of fresh culture medium (250 μl) was used to flush the wells through repeatedly pipetting to further collect the residual bacteria on the wells of the culture dish. The two bacterial solutions were pooled to make a total of 350 μl. The bacterial concentration was determined by the standard plating method immediately after the bacterial collection, and percentage of surviving bacteria was calculated. Polystyrene latex beads were purchased from Sigma-Aldrich (Saint Louis, Mo, USA) and used as negative controls.
All results were calculated from data of three independent experiments. T-test was used to assess statistical significance of differences in results of the antimicrobial effects. A P value of less than 0.05 (P < 0.05) was considered significant. The statistical tests were carried out and output to graphs using the Microsoft Excel (Microsoft Taiwan, Taipei, Taiwan) and SigmaPlot (Systat Software, Point Richmond, CA, USA) software.
Electron microscopic and Raman spectroscopic analysis
Killing of S. aureus by C150 and C200
Bacteria-killing experiment for other pathogens
Urbanization, population growth and heavy traveling enable infectious diseases to quickly spread worldwide from one local area. Photocatalyst has the potential for use in a variety of settings to reduce the transmission of pathogens in public environments. The emergence of increasingly virulent and antibiotic resistant pathogens in hospital settings [1, 3] provides another motivation for the development of alternative disinfection approaches using photocatalyst. There are several advantages to use the visible-light responsive photocatalyst such as titania. First, for safety consideration, visible light is a relatively safer light source as compared to UV irradiation . Exposure to UV light at the necessary levels, would cause great damage of skin and eye tissues for humans [33–35]; thus limiting the use of conventional UV activated TiO2 substrates in environments where humans would be exposed. The visible-light activated photocatalyst offers a perfect alternative for use as a disinfectant in public areas. Second, because TiO2 is a chemically stable and inert material, it could continuously exert antimicrobial action when illuminated by light. Third, the bactericidal activity can be switched on and off or modulated by controlling the light intensity. In addition, from efficiency point of view, commercial titania absorbs only the UV range estimated 2–3% of solar light impinging on the Earth's surface  when used for an outdoor setting. The advantages of the visible-light responsive photocatalyst might be complementary to existing disinfectants and provide the potential for developing a variety of alternative antimicrobial applications. To extend the light-absorption into visible-light range, doping with transition ions and/or anions (negative ions) is a commonly used method. By which it creates intra-band gap states close to the conduction or valence band edges that induces visible-light absorption at the sub-band gap energies [9, 36]. In some cases, the doped materials also are able to inhibit the charge recombination, thereby increase the photocatalytic activity [36–39]. Using such approach, many studies have shown to develop titania photocatalyst with antimicrobial activity in the visible-light range [13–15, 38–46]. In these studies, anions such as sulfur, nitrogen and carbon, and metal ions such as neodymium, tungsten, and platinum were used for doping titania. The photocatalytic- and antimicrobial-performance of these dopants are different because the various roles of the doped materials in trapping electrons and/or holes on the surface.
Besides photocatalytic activity, there are still other unidentified factors affect the antimicrobial activity. For example, catalysts may have similar photocatalytic activity but with different bactericidal performance as observed in the study using titania-coated nickel ferrite . Anatase-titania-coated nickel ferrite and brookite-titania-coated nickel ferrite have a similar photocatalytic reaction rate, while the former one has a superior bacterial-inactivation response . This indicates the physical properties such as bacterium-catalyst interactions might influence the antimicrobial outcome.
To analyze the influence of bacterium-catalyst interactions on the antimicrobial performance, we used C150 and C200 titania catalysts. Previously we found that both C150 and C200 samples have a similar visible light absorption pattern . C200 nanocrystals, however, contain mixed anatase and rutile phases that resulted in interface states in the mixed surface energy structures, as compared with uniform anatase phase structure of C150 nanocrystals . The distinct bacterial interaction behaviors of the C150 and C200, as observed in the SEM and Raman mapping in this study, presumably are attributed to the existence of different structural complexity. The interactions between TiO2 with biomolecules were rarely discussed. It was shown that various sol-gel treatments can change the property of TiO2 surfaces [47, 48]. It was also shown that different TiO2 crystal surfaces indeed have different affinities toward cellular protein fibronectin . In addition, carbon-coated TiO2 samples showed high affinity and high photoactivity towards organic compound methylene blue [48, 50]. Since bacterial surfaces express various organic components and proteins, it is not surprising that the bacteria would have a preferential interaction with specific catalyst. Using scanning electron microscopy and confocal Raman mapping techniques, here we successfully demonstrated that better bacterial-interaction is associated with better pathogen-killing performance when C200 samples were tested in bactericidal experiments.
In conclusion, we found that by generating mixed-phase TiO2 nanocrystals, the antibacterial activity of carbon-containing photocatalyst was significantly enhanced; and the photocatalysts can be used in the visible light settings. Although the bactericidal activity remains to be further improved and optimized, the unique property of C200 to interact with bacteria might provide a new perspective for developing more effective antibacterial photocatalysts.
The authors appreciate the financial support of National Science Council of Taiwan ROC under grant Nos. NSC 95-2314-B-320 -009 -MY3 and NSC 95-2120-M-259-003.
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