Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3
© Salaemae et al; licensee BioMed Central Ltd. 2010
Received: 26 April 2010
Accepted: 24 August 2010
Published: 24 August 2010
The dengue virus two-component protease NS2B/NS3 mediates processing of the viral polyprotein precursor and is therefore an important determinant of virus replication. The enzyme is now intensively studied with a view to the structure-based development of antiviral inhibitors. Although 3-dimensional structures have now been elucidated for a number of flaviviral proteases, enzyme-substrate interactions are characterized only to a limited extend. The high selectivity of the dengue virus protease for the polyprotein precursor offers the distinct advantage of designing inhibitors with exquisite specificity for the viral enzyme. To identify important determinants of substrate binding and catalysis in the active site of the dengue virus NS3 protease, nine residues, L115, D129, G133, T134, Y150, G151, N152, S163 and I165, located within the S1 and S2 pockets of the enzyme were targeted by alanine substitution mutagenesis and effects on enzyme activity were fluorometrically assayed.
Alanine substitutions were introduced by site-directed mutagenesis at residues L115, D129, G133, T134, Y150, G151, N152, S163 and I165 and recombinant proteins were purified from overexpressing E. coli. Effects of these substitutions on enzymatic activity of the NS3 protease were assayed by fluorescence release from the synthetic model substrate GRR-amc and kinetic parameters Km, kcat and kcat/Km were determined.
Kinetic data for mutant derivatives in the active site of the dengue virus NS3 protease were essentially in agreement with a functional role of the selected residues for substrate binding and/or catalysis. Only the L115A mutant displayed activity comparable to the wild-type enzyme, whereas mutation of residues Y150 and G151 to alanine completely abrogated enzyme activity. A G133A mutant had an approximately 10-fold reduced catalytic efficiency thus suggesting a critical role for this residue seemingly as part of the oxyanion binding hole.
Kinetic data obtained for mutants in the NS3 protease have confirmed predictions for the conformation of the active site S1 and S2 pockets based on earlier observations. The data presented herein will be useful to further explore structure-activity relationships of the flaviviral proteases important for the structure-guided design of novel antiviral therapeutics.
Dengue virus, a member of the Flaviviridae family, is a small, spherical, enveloped, positive single strand RNA virus that is transmitted to humans by mosquitoes of the species Stegomyia aegypti (formerly Aedes). All 4 serotypes of the virus (DEN-1, 2, 3 and 4) can cause a spectrum of clinical symptoms including mild dengue fever (DF) and more severe forms of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) [1, 2]. An increase of geographical spread, incidence and severity of diseases over the past decade has now stimulated intensive efforts to develop effective antiviral therapeutics which are eventually useful for the prevention and cure of dengue virus infections. The development of small molecule drugs directed at inhibition of replication and maturation of the virus is now considered as promising route for the treatment of acute dengue diseases [for review see[3–5]and references herein].
The dengue virus NS3 protease, a member of the flavivirin enzyme family (EC 126.96.36.199), is located in the N-terminal 184 residues of the multifunctional 69 kDa NS3 protein and contains a functional catalytic triad consisting of H51, D75 and S135 (in DEN-2) . In addition to the serine protease, the NS3 protein contains enzymatic activities of a nucleoside triphosphatase, a 5' - RNA triphosphatase (RTPase) and a RNA - stimulated RNA helicase [7, 8]. The NS3 protease catalyses the post-translational cleavage of the viral polyprotein precursor in the non-structural region at the NS2A/NS2B, NS2B/NS3, NS3/NS4A and NS4B/NS5 sites and at additional sites within the viral capsid protein, NS2A, NS4A and within a C-terminal region of NS3 itself [9–13]. The overall conformation of the dengue virus NS3 protease displays the β-barrel conformation typical for serine proteases, although the viral enzyme appears to possess higher compactness with short or absent loop structures and a relatively shallow substrate binding site .
The presence of a small hydrophilic core segment of approximately 40 residues, commonly designated NS2B(H), within the small 14 kDa NS2B cofactor is required for optimal activity of the NS3 protease [15–17]. Proteolytic autoprocessing at the NS2B/NS3 site generates a non-covalent adduct between NS2B(H) and NS3 which is catalytically active with substrates supplied in trans cleavage reactions .
Detailed substrate specificity studies have established that the cleavage junctions in the viral polyprotein consist of pairs of dibasic amino acids such as RR, RK and KR at the P1 and P2 positions. Small, non-branched amino acids such as S are preferred at the P1' position of the dengue virus cleavage site, whereas the preferred P1' residue of the WNV NS3 protease is G [19–21].
Theoretical molecular interactions between the active site of the NS3 protease and the peptide substrate were largely consistent with data obtained from substrate profiling studies . Crystallographic studies of flaviviral proteases including the West Nile Virus (WNV) and dengue virus in complex with a partial NS2B cofactor and substrate-mimetic inhibitors such as aprotonin have provided evidence for major structural reorganizations of the active site pockets caused by insertion of a β-barrel of the NS2B cofactor and an "induced fit" mechanism of catalysis in the presence of authentic protein substrates . Based on a homology-modelled structure of the WNV NS3 protease, residues within the S1 and S2 pockets critical for enzyme-substrate interaction were identified by analysis of catalytic activity of mutant proteases with a synthetic peptide substrate . Structural data obtained recently for a WNV NS2B-NS3pro protease in complex with a substrate-based tripeptide inhibitor have revealed a catalytically competent oxyanion binding site formed by two residues, G133 and S135, and substitution of the active-site nucleophile serine by alanine does not result in a disruption of the oxyanion conformation . It is noteworthy that also in the presence of ligands without a P1' residue the active conformation of the oxyanion hole is adopted by the viral protease.
A high conservation of sequences within the faviviral proteases suggests that specificity characteristics found for the WNV protease could also be of relevance for the closely related dengue virus NS3 protease. Despite their overall similarities, the NS3 proteases from dengue virus and WNV exhibit different substrate specificities, suggesting a distinct organization of their respective active site conformations .
In analogy to procedures previously described for the enzyme from WNV, we have identified key residues for substrate binding and catalysis of the dengue virus NS3 protease by alanine substitution mutagenesis and assay of the recombinant mutant enzymes with a synthetic model substrate. In fact, an earlier study has described extensive mutagenesis within the dengue virus NS3 protease for ultraconserved residues among flaviviral proteases and these residues were putatively involved in catalysis or substrate binding . However, activity of the mutant proteases was assayed by SDS-PAGE analysis of autoproteolytic cleavage of the NS2B-NS3 precursor in vivo. Although this approach yielded semiquantitative data for activity of the mutant enzymes, it did not provide precise numerical values for the kinetic activity of the mutant proteases with substrates supplied for trans cleavage reactions. Moreover, a number of residues such as L115, S163 and I165 have not been included in that investigation as their possible role for enzyme activity was suggested later by data from structural experiments [14, 23]. Therefore, the changes in catalytic efficiency which we have observed in the context of amino acid exchanges could contribute to a refined model of substrate specificity and active site conformation for the dengue virus NS3 protease.
Construction of active site mutants of the dengue virus NS2B(H)-NS3pro protease
Plasmid DNA encoding the NS2B(H)-NS3pro precursor of dengue virus serotype 2 strain 16681 cloned in the pTrcHisA expression vector (Invitrogen) containing residues 48-95 followed by residues 121-130 and the N-terminal 180 amino acids of the NS3 protein was used as template for site-directed mutagenesis by using the QuickChange site-directed mutagenesis kit (Stratagene) as described earlier . All synthetic oligonucleotides were purchased from Proligo Pty., Singapore. Mutagenic primers were designed to induce alanine substitutions at positions L115, D129, G133, T134, Y150, G151, N152, S163 and I165 within the NS3pro protein. PCR reactions were carried out by using an automated thermal cycler (Perkin Elmer). Additional restriction sites were incorporated in the primer sequence for screening purposes. A catalytically inactive S135A mutant of NS3pro was used as negative control. Plasmid DNA obtained from recombinant clones was subjected to DNA sequence analysis by using an ABI PRISM™Dye Terminator Cycle Sequencing kit on a model 377 DNA sequencer (Perkin-Elmer, Norwalk, USA). No mutations were found to be present in the plasmid samples at non-targeted sites.
Expression and purification of the mutant NS2B(H)-NS3pro proteases
Plasmid DNA containing recombinant sequences of the dengue virus NS3pro mutants was transformed in E. coli C41(DE3) host cells and cultures were incubated at 37°C in the presence of 0.1 mM IPTG for 8 hours. Protein complexes were purified from inclusion bodies by a two-step procedure using a Hitrap chelating column (Pharmacia) and a Superdex 75 HR 10/300 gel filtration column (Pharmacia) as described earlier . Purification was performed under denaturing conditions in the presence of 8 M urea and purified proteins were refolded by step-wise dialysis using Spectra/Por 6 regenerated cellulose dialysis membranes (Spectrum Laboratories). Samples were concentrated by using Amicon Ultra-15 centrifugal filter devices (Millipore). Protein concentrations were determined by using a BioRad protein assay dye reagent kit based on the Bradford method with bovine serum album (Sigma Chemistry) as standard. Protein samples were analyzed on 15% SDS-PAGE gels using a Mini-Protein III electrophoresis system (BioRad). Preparations of the refolded enzymes were stored at -20°C in 0.1 M Tris-HCl, pH 9.0, 50% (v/v) glycerol for up to 1 week.
Assay of enzymatic activity
Enzymatic activity of the NS2B(H)-NS3pro recombinant proteases was assayed with the fluorogenic peptide substrate, tBoc-Gly-Arg-Arg-4-methylcoumaryl-7-amide (Peptides International), in 50 mM Tris-HCl, pH 9.0, 20% (v/v) glycerol, by using an automated microtiter plate fluorescence reader (Perkin Elmer) at excitation wavelength λ = 355 nm and emission wavelength λ = 460 nm as described previously . Briefly, reactions were initiated by mixing the substrate solution with the enzyme and fluorescence signals were recorded at 5 minutes intervals over a period of 20 min at 37°C. Initial velocities were corrected for inner filter effects as described in the literature . Concentrations of proteases used in the assay were dependent on activity and were varied between 0.15 μM and 3.0 μM, substrate concentrations were in the range between 12.5 μM and 1.0 mM. Fluorescence signals were converted to product formation by comparison with standard amounts of amc (Sigma Chemistry). Kinetic parameters, Km and Vmax, were obtained from measurement of corrected velocities and Michaelis-Menten kinetics, v = Vmax [S]/[S]+Km, were transformed into double-reciprocal Lineweaver-Burk plots by non-linear regression analysis using the GraphPad Prism 4 software. Three independent experiments were carried out for each set of data points and data are reported as mean ± SEM by one-way analysis of variance calculated by using GraphPad InStat 3 software. Standard deviations of the reported numerical values were < 10%.
Results and discussion
Construction, expression and purification of active site mutants in the dengue virus NS3 protease
Analysis of autoproteolysis as described earlier for mutants in the NS2B(H) activation sequence  revealed that the Y150A and G151A mutants were completely inactive with no products of self-cleavage detectable (data not shown).
Kinetic analysis of active-site mutations
G151 and G133
Contrary to our expectations, alanine substitution of G151 yielded an enzyme with completely abolished activity. G151 is invariantly present in the NS3 sequences of known flaviviruses and we speculated that this residue might play a dual role in the stabilization of the tetrahedral transition state intermediate formed at S135 during substrate cleavage and to maintain structural stability of the E2-F2 strands in the protease fold . G133 is part of the ultraconserved GxSGxP motif found in flaviviral NS3 sequences and most likely determines the optimal size and generates the catalytically competent conformation of the oxyanion hole for the accommodation of the respective substrate . Earlier reports have proposed that substrate-free conformations of the NS2B-NS3pro enzyme contain a flipped peptide bond between T132 and G133 which abrogates the active conformation of the oxyanion hole and that formation of the active oxyanion hole by an induced fit mechanism requires the presence of authentic substrates containing a P1' residue [14, 23]. However, it was demonstrated recently that the active conformation of the oxyanion hole in WNV NS3 protease is maintained also in the presence of inhibitors without a P1' residue .
Alanine substitution of the G133 residue resulted in a NS3 protease with only approximately 10-fold reduced catalytic efficiency when compared with the wild-type enzyme, thus suggesting some degree of conformational freedom at this position.
Substitution of Y150 by alanine yielded a NS3 protease with completely abrogated activity, a finding in agreement with earlier mutagenesis data for this residue from WNV and dengue virus [24, 25]. It was suggested that Y150 could primarily stabilize the positively charged side chain of the P1 arginine by an aromatic π - cation interaction. An additional role for Y150 could be the structural stabilization of the E2 strand in the C-terminal β-barrel of the NS3 protein.
This position is structurally homologous to residue 226 of chymotrypsin which is part of the portal to the substrate binding pocket and is conserved in all flavivirus proteases. It was proposed earlier that G153 and S163 form a bulkier entry to the substrate binding pocket . Mutation of S163 to alanine in the WNV NS3 protease generated an inactive enzyme , whereas we have found a 12-fold reduction in catalytic efficiency for the dengue virus S163A mutant. This residue could play a role in substrate binding by formation of a hydrogen bond with the substrate P1 arginine.
Substitution of the invariant residue D129 by alanine induced a large (39-fold) decrease in catalytic efficiency. Chappell et al. have reported a 17-fold increase in Km for the D129A mutant of the WNV protease, whereas we have seen a 2.3-fold increase in Km and a 16-fold decrease in kcat. The precise role of this residue requires further experimental analysis, however, the possibility exists that D129 participates both in substrate binding by providing salt bridge or hydrogen bond interactions with the substrate P1 arginine as well as in the catalytic mechanism of the NS3 protease.
Alanine substitutions of this residue had only minor effects on enzymatic activity as demonstrated by a 2.2-fold decrease in catalytic efficiency. T134 could provide a weak interaction with the P1 arginine, presumably via a hydrogen bond from its hydroxyl group.
A V115F mutant of the WNV NS3 protease was enzymatically inactive most likely due to size restrictions which prevented the substrate from occupying the S1 pocket , however, in the dengue virus NS3 protease L115 appears to be located at a position relatively remote from the S1 pocket (Fig. 2) . For the L115A mutant of dengue virus NS3 protease, we have observed even a marginal increase in catalytic efficiency suggesting that a smaller side chain could provide additional flexibility for the accommodation of the P1 substrate.
In contrast to findings with the WNV virus NS3 protease, the N152A mutation did not completely inactivate the dengue virus enzyme but resulted in a substantial 60-fold reduction of catalytic efficiency. N152 is part of the S2 subsite and presumably provides an interaction with the side chain of the P2 substrate via hydrogen bonding .
The role of this residue was not investigated in previous studies although it is conserved throughout the flavivirus NS3 proteases and was predicted to line the S1 pocket. Mutation of this residue to alanine removes the bulky side chain and results in a drastic increase in Km thus suggesting a function in substrate binding rather than catalysis, conceivably by a reduction of the size of the S1 pocket.
Taken together, these results have largely confirmed previous predictions for active site residues of the dengue virus NS3 protease and therefore suggest that the residues selected in this study have probably a major function for substrate binding and catalysis. In addition to existing structural data we have further obtained enzymatic evidence to suggest that the residues G133 and G151 play a role in the catalytic mechanism of the dengue virus NS3 protease, presumably as structural elements of the oxyanion binding hole. Although our findings for the dengue virus NS3 protease are essentially in agreement with previous data for the WNV NS3 protease, we have observed that a number of residues such as S163, N152 and I165 display distinct effects on enzyme activity when substituted by alanine. These differences could relate to subtle structural alterations in the structures of the WNV and dengue virus proteases . Moreover, differences in substrate specificity were recently explained by alteration of the substrate binding pockets in the S2-S4 region by dissimilarities in NS2B complexation between the dengue virus and WNV NS3 proteases . It is also noteworthy that the S1 and S2 pockets of a proteolytic enzyme may not be the sole determinants of specificity . Nevertheless we believe that the S1 and S2 residues identified in this study may represent ideal targets for the design of antiviral inhibitors against NS3 serine proteases of dengue virus and possibly other flaviviruses.
The design of inhibitors against the flaviviral NS3 serine proteases requires a precise knowledge of the structural determinants of substrate binding and catalysis. In this study we have re-visited the conformational properties of the dengue virus NS3 protease active site by a structure-guided mutagenesis approach of nine residues located within the S1 and S2 binding pockets. Of these, all with exception of L115 had prominent effects on enzyme catalysis and therefore represent important functional determinants for substrate specificity. To the best of our knowledge, this study describes for the first time a kinetic analysis of mutations in the dengue virus NS3 protease by a trans cleavage assay with a synthetic peptide model substrate.
These structural requirements can be utilized in informed drug discovery programs aiming at the discovery of selective inhibitors against the flaviviral NS3 proteases.
dengue virus serotype 2
dengue hemorrhagic fever
dengue shock syndrome
- NS2B and NS3:
dengue virus non-structural viral proteins 2B and 3: respectively
the protease domain of the NS3 protein
the central hydrophilic activation domain of the NS2B protein
polyacrylamide gel electrophoresis
West Nile virus.
We thank Prof. Dr. Jarl E. S. Wikberg, Dept. of Pharmaceutical Biosciences, Uppsala University, Sweden, for critical discussions. Anchalee Nirachanon is acknowledged for her excellent secretarial assistance. A PhD scholarship to MJ from the University of Malakand, Government of Pakistan, is gratefully acknowledged. This work was supported by grant BRG490008 (to GK) from the Thailand Research Fund (TRF).
- Halstead SB: Pathogenesis of dengue: challenges to molecular biology. Science. 1988, 239: 476-481. 10.1126/science.3277268.View ArticlePubMedGoogle Scholar
- Monath TP: Dengue: the risk to developed and developing countries. Proc Natl Acad Sci USA. 1994, 91: 2395-2400. 10.1073/pnas.91.7.2395.PubMed CentralView ArticlePubMedGoogle Scholar
- Melino S, Paci M: Progress for dengue virus diseases. Towards the NS2B-NS3pro inhibition for a therapeutic-based approach. FEBS J. 2007, 274: 2986-3002. 10.1111/j.1742-4658.2007.05831.x.View ArticlePubMedGoogle Scholar
- Sampath A, Padmanabhan R: Molecular targets for flavivirus drug discovery. Antiviral Res. 2009, 81: 6-15. 10.1016/j.antiviral.2008.08.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Lescar J, Luo D, Xu T, Sampath A, Lim SP, Canard B, Vasudevan SG: Towards the design of antiviral inhibitors against flaviviruses: The case for the multifunctional NS3 protein from Dengue virus as a target. Antiviral Res. 2008, 80: 94-101. 10.1016/j.antiviral.2008.07.001.View ArticlePubMedGoogle Scholar
- Bazaan JF, Fletterick RJ: Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology. 1989, 171: 637-639. 10.1016/0042-6822(89)90639-9.View ArticleGoogle Scholar
- Bartelma G, Padmanabhan R: Expression, purification and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 non-structural protein 3. Virology. 2002, 299: 122-133. 10.1006/viro.2002.1504.View ArticlePubMedGoogle Scholar
- Luo D, Xu T, Hunke C, Gruber G, Vasudevan SG, Lescar J: Crystal structure of the NS3 protease-helicase from dengue virus. J Virol. 2008, 82: 173-183. 10.1128/JVI.01788-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Chambers TJ, Weir RC, Grakoui A, McCourt DW, Bazan JF, Fletterick RJ, Rice CM: Evidence that the N-terminal domain of non-structural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proc Natl Acad Sci USA. 1990, 87: 8898-8902. 10.1073/pnas.87.22.8898.PubMed CentralView ArticlePubMedGoogle Scholar
- Lin C, Amberg SM, Chambers TJ, Rice CM: Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site. J Virol. 1993, 67: 2327-2335.PubMed CentralPubMedGoogle Scholar
- Lobigs M: Flavivirus premembrane protein cleavage and spike heterodimer secretion require the function of the viral proteinase NS3. Proc Natl Acad Sci USA. 1993, 90: 6218-6222. 10.1073/pnas.90.13.6218.PubMed CentralView ArticlePubMedGoogle Scholar
- Preugschat F, Yao CW, Strauss JH: In vitro processing of dengue virus type 2 non-structural proteins NS2A, NS2B, and NS3. J Virol. 1990, 64: 4364-4374.PubMed CentralPubMedGoogle Scholar
- Teo KF, Wright PJ: Internal proteolysis of the NS3 protein specified by dengue virus 2. J Gen Virol. 1997, 78 (Pt 2): 337-341.View ArticlePubMedGoogle Scholar
- Erbel P, Schiering N, D'Arcy A, Renatus M, Kroemer M, Lim SP, Yin Z, Keller TH, Vasudevan SG, Hommel U: Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol. 2006, 13: 372-373. 10.1038/nsmb1073.View ArticlePubMedGoogle Scholar
- Niyomrattanakit P, Winoyanuwattikun P, Chanprapaph S, Angsuthanasombat C, Panyim S, Katzenmeier G: Identification of residues in the dengue virus type 2 NS2B cofactor that are critical for NS3 protease activation. J Virol. 2004, 78: 13708-13716. 10.1128/JVI.78.24.13708-13716.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Falgout B, Miller RH, Lai CJ: Deletion analysis of dengue virus type 4 non-structural protein NS2B: Identification of a domain required for NS2B-NS3 protease activity. J Virol. 1993, 67: 2034-2042.PubMed CentralPubMedGoogle Scholar
- Chappell KJ, Stoermer MJ, Fairlie DP, Young PR: Mutagenesis of the West Nile virus NS2B cofactor domain reveals two regions essential for protease activity. J Gen Virol. 2008, 89: 1010-1014. 10.1099/vir.0.83447-0.View ArticlePubMedGoogle Scholar
- Yusof R, Clum S, Wetzel M, Murthy HM, Padmanabhan R: Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro. J Biol Chem. 2000, 275: 9963-9969. 10.1074/jbc.275.14.9963.View ArticlePubMedGoogle Scholar
- Li J, Lim SP, Beer D, Patel V, Wen D, Tumanut C, Tully DC, Williams JA, Jiricek J, Priestle JP, Harris JL, Vasudevan SG: Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetra- and octa-peptide substrate libraries. J Biol Chem. 2005, 280: 28766-28774. 10.1074/jbc.M500588200.View ArticlePubMedGoogle Scholar
- Niyomrattanakit P, Yahorova S, Mutule I, Mutulis F, Petrovska R, Prusis P, Katzenmeier G, Wikberg JE: Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides. Biochem J. 2006, 397: 203-211. 10.1042/BJ20051767.PubMed CentralView ArticlePubMedGoogle Scholar
- Shiryaev SA, Kozlov IA, Ratnikov BI, Smith JW, Lebl M, Strongin AY: Cleavage preferences distinguishes the two-component NS2B-NS3 serine proteinases of Dengue and West Nile viruses. Biochem J. 2007, 401: 743-752. 10.1042/BJ20061136.PubMed CentralView ArticlePubMedGoogle Scholar
- Chappell KJ, Stoermer MJ, Fairlie DP, Young PR: Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modelling, protease mutagenesis, and kinetic studies. J Biol Chem. 2006, 281: 38448-38458. 10.1074/jbc.M607641200.View ArticlePubMedGoogle Scholar
- Aleshin AE, Shiryaev SA, Strongin AY, Liddington RC: Structural evidence for regulation and specificity of flaviviral proteases and evolution of the Flaviviridae fold. Protein Sci. 2007, 16: 795-806. 10.1110/ps.072753207.PubMed CentralView ArticlePubMedGoogle Scholar
- Chappell KJ, Nall TA, Stoermer MJ, Fang NX, Tyndall JD, Fairlie DP, Young PR: Site-directed mutagenesis and kinetic studies of the West Nile Virus NS3 protease identify key enzyme-substrate interactions. J Biol Chem. 2005, 280: 2896-2903. 10.1074/jbc.M409931200.View ArticlePubMedGoogle Scholar
- Robin G, Chappell K, Stoermer MJ, Hu SH, Young PR, Fairlie DP, Martin JL: Structure of West Nile virus NS3 protease: Ligand stabilization of the catalytic conformation. J Mol Biol. 2009, 385: 1568-1577. 10.1016/j.jmb.2008.11.026.View ArticlePubMedGoogle Scholar
- Valle RPC, Falgout B: Mutagenesis of the NS3 protease of Dengue virus type 2. J Virol. 1998, 72: 624-632.PubMed CentralPubMedGoogle Scholar
- Liu Y, Kati W, Chen CM, Tripathi R, Molla A, Kohlbrenner W: Use of a fluorescence plate reader for measuring kinetic parameters with inner filter effect correction. Anal Biochem. 1999, 267: 331-335. 10.1006/abio.1998.3014.View ArticlePubMedGoogle Scholar
- Perona JJ, Craik CS: Structural basis of substrate specificity in the serine proteases. Protein Sci. 1995, 4: 1331-1342.Google Scholar
- Shiryaev SA, Ratnikov BI, Aleshin AE, Kozlow IA, Nelson NA, Lebl M, Smith JW, Liddington RC, Strongin AY: Switching the substrate specificity of the two-component NS2B-NS3 flavivirus proteinase by structure-based mutagenesis. J Virol. 2007, 81: 4501-4509. 10.1128/JVI.02719-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Mueller N, Yon C, Ganesh VK, Padmanabhan R: Characterization of the West Nile virus substrate specificity and inhibitors. Int J Biochem Cell Biol. 2007, 39 (3): 606-614. 10.1016/j.biocel.2006.10.025.View ArticlePubMedGoogle Scholar
- Schellenberger V, Turck CW, Rutter WJ: Role of the S' subsites in serine protease catalysis. Active-site mapping of rat chymotrypsin, rat trypsin, alpha-lytic protease, and cercarial protease from Schistosoma mansoni. Biochemistry. 1994, 33: 4251-4257. 10.1021/bi00180a020.View ArticlePubMedGoogle Scholar
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