Drosophila king tubby (ktub) mediates light-induced rhodopsin endocytosis and retinal degeneration
© Chen et al.; licensee BioMed Central Ltd. 2012
Received: 4 November 2012
Accepted: 4 December 2012
Published: 10 December 2012
The tubby (tub) and tubby-like protein (tulp) genes encode a small family of proteins found in many organisms. Previous studies have shown that TUB and TULP genes in mammalian involve in obesity, neural development, and retinal degeneration. The purpose of this study was to investigate the role of Drosophila king tubby (ktub) in rhodopsin 1 (Rh1) endocytosis and retinal degeneration upon light stimulation.
Drosophila ktub mutants were generated using imprecise excision. Wild type and mutant flies were raised in dark or constant light conditions. After a period of light stimulation, retinas were dissected, fixed and stained with anti-Rh1 antibody to reveal Rh1 endocytosis. Confocal and transmission electron microscope were used to examine the retinal degeneration. Immunocytochemical analysis shows that Ktub is expressed in the rhabdomere domain under dark conditions. When flies receive light stimulation, the Ktub translocates from the rhabdomere to the cytoplasm and the nucleus of the photoreceptor cells. Wild type photoreceptors form Rh1-immunopositive large vesicles (RLVs) shortly after light stimulation. In light-induced ktub mutants, the majority of Rh1 remains at the rhabdomere, and only a few RLVs appear in the cytoplasm of photoreceptor cells. Mutation of norpA allele causes massive Rh1 endocytosis in light stimulation. In ktub and norpA double mutants, however, Rh1 endocytosis is blocked under light stimulation. This study also shows that ktub and norpA double mutants rescue the light-induced norpA retinal degeneration. Deletion constructs further demonstrate that the Tubby domain of the Ktub protein participates in an important role in Rh1 endocytosis.
The results in this study delimit the novel function of Ktub in Rh1 endocytosis and retinal degeneration.
KeywordsKing-tubby Rhabdomere Phototransduction Endocytosis Retinal degeneration
The tubby (tub) and tubby-like protein (tulp) genes encode a small family of proteins found in many organisms, including Drosophila, C. elegans[2, 3], Gallus, Arabidopsis and other plants. Four members of Tubby proteins are in mammals, including TUB, TULP1, TULP2 and TULP3[7, 8]. Mutation in mouse tubby gene leads to photoreceptor and cochlear degeneration and adult-onset obesity[9–11]. Target deletion of tulp1 in mice causes photoreceptor cell degeneration[12, 13]. Mutation of tulp3−/− in mice causes defects in neural tube development, increases neuronal apoptosis, and eventually leads to embryonic lethality. Human mutations of TULP1 gene result in retinitis pigmentosa, an inherited disease that typically causes retinal degeneration[15–17]. Taken together, these studies suggest an important role of Tub family proteins in retinal development and maintenance. Structural analysis indicates that the C-terminus of TULP proteins are highly conserved, and contain a DNA binding domain. The N-terminus of TULP proteins contain the remnants of the transactivation domains of many transcription factors, suggesting that TULPs may act as transcription factors[18, 19]. Subsequent studies have shown that TULPs bind to actin and Dynamin-1, suggesting their function in regulating vesicle transport in photoreceptor cells[20, 21]. Mutation of tulp1 in mice causes mislocalization of rhodopsin in photoreceptor cells and abnormal formation of photoreceptor synapse[22, 23]. According to recent studies, Tulps have an extracellular function in which they act as phagocytosis ligands for retinal pigment epithelium[24, 25]. The role of Tulp as phagocytosis ligand occurs through binding to the MerTK, a TAM receptor tyrosine kinase subfamily. Together, these studies have shown important functions of Tulps in multicellular organisms. However the molecular and cellular functions of tubby family proteins remain obscure. The Drosophila visual system is an excellent model for studying retinal degeneration[27–29]. The Drosophila genome contains one gene, king tubby (ktub), belonging to the tub gene family. Immunocytochemical study has indicated that ktub is expressed in the developing nervous system, suggesting its role in neural development. Whether ktub participates in retinal degeneration and mediates phototransduction cascade remains unclear. Drosophila photoreceptor cells contain specialized portions of the plasma membrane, called the rhabdomeres. Each rhabdomere consists of numerous tightly packed microvilli, rhodopsin photopigments, and other components of the phototransduction cascade[30–32]. The phototransduction cascade in Drosophila begins with the light activation of rhodopsin (Rh1). Once activated, Rh1 binds to heterotrimeric G protein, which catalyzes the exchange of GDP for GTP on the Gα subunit (Gαq). The Gαq subunit then activates retinal-specific phospholipase C and causes the opening of the cation-specific channels Trp and Trpl. This eventually leads to the depolarization of the photoreceptor cell and neurotransmitter release. After light activation, rhodopsin kinase and arrestin inactivate the rhodopsin activity[33–35]. Arrestin and AP-2 are critical factors for receptor into clathrin mediate endocytosis. Studies in Drosophila have demonstrated that visual arrestin (Arr1) is essential for light-induced Rh1 internalization and Arr2 is involved in rhodopsin endocytosis under certain pathological situations[38–40].
This article studies how Ktub participates in Drosophila phototransduction and retinal degeneration. Results show that subcellular localization of Ktub in adult photoreceptor cells is light-dependent. In ktub mutant, rhodopsin endocytosis is blocked under light conditions. In addition, retinal degeneration is evident in ktub mutant flies reared in constant light. In norpA mutant, massive endocytotic rhodopsin vesicles appear in the cytoplasm. However, the rhodopsin vesicles appear less in the cytoplasm of norpA and ktub double mutant. To further investigate what domain in Ktub protein is involved in rhodopsin endocytosis, this study uses deletion constructs to examine its ability to mediate endocytosis. Results show that the C-terminal Tubby domain is required for endocytosis. Taken together, these results provide new evidence showing that the Ktub protein is required for mediating rhodopsin endocytosis and retinal degeneration.
Drosophila stocks and transgenic constructions
Drosophila melanogaster w 1118 was used as wild type. P-element insertion fly, P(GSV6)17325/SM was obtained from the Szeged Drosophila stock center and was used for imprecise excision to isolate ktub mutants. norpA 33 , Df (2R) ED3791, and rh1-Gal4 were obtained from the Bloomington stock center. All flies were reared on standard corn meal agar media at 25°C in dark, 12D/12 L light or constant-light condition. The ambient light used to create light-induced condition was approximately 500 lux. To make transgenic flies that expressed full-length, N-terminus (N-ktub) and C-terminus (C-ktub) of Ktub protein, we amplified ktub cDNA by PfuTurbo DNA polymerase from EST clone, RE38560, using the following primers: ktub (5’-ATGTCCGGAATCAACAGTCGTAATCAG-3’, 5’-TCACTC GCAGGCTATTT TGC-3’); N-ktub (5’-ATGTCCGGAATCAACAGTCGTAATCAG-3’, 5’-ATTGCCGATGACATCTCCCTCGGAC-3’); C-ktub (5’-ATCGACCAGTTCGTGATGC AAC-3’, 5’-TCACTCGCAGGCTATTTTGC-3’). The PCR fragments were subcloned into pUAST-Flag expression vector to make pUAST-Flag-ktub, pUAST- Flag- N-ktub, and pUAST- Flag-C-ktub. All constructions were verified by DNA sequencing before germ-line transformation. P-element mediated germ-line transformation produced more than three independent lines. The transgenic lines were crossed to rh1-Gal4 for further analysis.
Generation of ktub mutant fly by P-element imprecise excision
To generate ktub mutants, we crossed P(GSV6)17325/SM to a transposase expressing line (Δ2–3) to induce P-element excision. After imprecise excision, flies with white eye were crossed to Df (2R) ED3791 to isolate the ktub /Df (2R) ED3791 adult flies. To identify the mutation site, two primers: 5’–GGCAATTTCAATCGAATTTACC-3’ and 5’-ATCGAA GTAACTCGAAGGACCC-3’ were used to amplify the 5’ region of the ktub gene. A total of 200 P-excision lines were screened to obtain six lines with DNA deletion in the ktub gene. DNA sequence confirmed two alleles, ktub 35-3 and ktub 115-4 , have deleted the ATG translation start site, and these two alleles were used for further analysis.
Antibody production and Western blotting
To generate antibody against Ktub protein, PCR was used to amplify ktub fragments by two primers: 5’-ATGGAGGCCTACATCCGGCAGAAGAG-3’, 5’-TCACTCGCAGGCTATTT TGCCATCGA- 3’. The PCR products were cloned into pQE-31 vector (Qiagen, Valencia, CA). After IPTG induction, a 56 kDa Ktub recombinant protein was isolated from E. coli and used as antigen to inject rabbit. After several boosts, the serum was collected to test its immunoreactivity. For Western blotting, adult eyes were collected and homogenized with a homogenization buffer (50 mM HEPES, 50 mM KCl, 1 mM EGTA, 1 mM MgCl2, 10% Glycerol) with protease inhibitors. The cell extract was then centrifuged at 1500 g for 10 min at 4°C and subjected to SDS-PAGE to separate the protein. SDS-PAGE and Western blotting were performed with slight modification from previous studies. After electrophoresis, the proteins were transferred to a PVDF membrane. To perform immune blotting, the membrane was blocked with 5% non-fat milk in TBST (10 mM Tris, pH7.4, 150 mM NaCl, with 0.1% Tween20). The membrane was then incubated with anti-Ktub antiserum (1:2000) at 4°C overnight. The following day, the membrane was washed three times with TBST and then incubated with peroxidase conjugated goat anti-rabbit IgG (1:10000). After incubation with the secondary antibody, the membrane was washed and processed for chemiluminescent reaction (Milipore, Billerica MA). The signals were detected with a CCD camera (Fuji film, Japan).
To perform immunohistochemical staining, dissected eyes were fixed in 4% paraformaldehyde for 20 minutes. After three washes and blocking, the eyes were incubated with primary antibody. The primary antibodies used in this study included rabbit anti-Ktub (1:500) and mouse anti-Rh1 (4C5) from Developmental Studies Hybridoma Bank (1:100). Rhodamine or FITC conjugated phalloidin (Sigma-Aldrich, St. Louis, MO), which stained the F-actin, was used to label the cell boundary. Stained eye discs were washed three times with PBST (137 mM NaCl, 2.68 mM KCl, 10 mM Na2HPO4, 1.7 mM KH2PO4, pH7.2 with 0.2% TritonX-100), and then incubated with secondary antibodies. The secondary antibodies used in this study were conjugated with Alex 488 (Invitrogen Molecular Probes, Carlsbad, CA), Texas Red, or Cy5 (Jackson Immuno Research Lab. West Grove, PA). After three washes, eyes were mounted in a mounting medium (0.25% n-propyl gallate, 50% glycerol in PBS, pH 8.6) and examined using a Zeiss LSM 510 confocal microscope. Images were processed using Adobe Photoshop 6.0 software.
Transmission electron microscopy was performed as previously described. Flies were injected with a fixative (2% paraformaldehyde and 2% glutaraldehyde in 0.1 M cacodylate buffer). After dissection, the eyes were incubated in fixative for another 2 hours and postfixed with 2% OsO4 in 0.1 M cacodylate buffer at 4°C. The eyes were subjected to series dehydration with alcohol and embedded in Epox-812 (EMS). The tissues were then sectioned using a Reichert ultramicrotome and observed using a Tecnai spirit G2 (FEI) transmission electron microscope.
Isolation of ktub mutants and their phenotypic analysis
The subcellular localization of Ktub in Drosophila eye is light-dependent
Knockdown ktub expression resulted in light-dependent retinal degeneration
Drosophila ktub participates in rhodopsin endocytosis
ktub rescues norpA mediated endocytosis and retinal degeneration
Tubby domain is critical for mediating rhodopsin endocytosis
The results of this study demonstrate that Drosophila ktub plays an important role in mediating Rh1 endocytosis. Immunocytochemical analysis reveals that Ktub is primarily located in the rhabdomeric domain in the dark. When the eye was exposed to light, the Ktub protein was immediately translocalized from the rhabdomere to the cytoplasm of the photoreceptor cells. This study further demonstrated that the subcellular expression pattern of the Ktub is correlated with Rh1 expression, which is also internalized upon light stimulation. The correlation of Ktub and Rh1 expression in photoreceptor cells suggests that these two molecules play similar roles during phototransduction. To determine whether Ktub regulates Rh1 internalization, we generated ktub mutants and assayed the role of Ktub in Rh1 endocytosis. Light-reared ktub mutant flies had significantly fewer RLVs in the cytoplasm of the photoreceptor than the wild type. In addition, light-stimulated ktub mutant flies also exhibited a severe retinal degeneration phenotype. To further demonstrate the role of ktub in endocytosis, we examined its ability to suppress norpA-mediated endocytosis. Results clearly demonstrate that ktub mutant blocked norpA-mediated endocytosis and retinal degeneration. Previous research has intensively studied the functions of Tub proteins in vertebrate systems. Several studies have shown that Tub and Tulp proteins are involved in retinal degeneration, obesity, and protein trafficking . Studies also show that retinal degeneration in tubby mice is regulated by caspase-3 mediated pathway. Currently, we do not know whether the retinal degeneration in ktub mutant is mediated by caspase-3 mediated pathway. However, previous studies show that the retinal degeneration in norpA mutant is not regulated by caspase mediated pathway. The results in this study find that ktub mutant could rescue norpA mutant phenotype suggesting that the retinal degeneration in ktub mutant is not through caspase mediated pathway. Thus, it will be interested to study the molecular mechanism of Ktub in mediated retinal degeneration. This study shows that Ktub protein was mainly localized in the rhabdomeric microvilli of dark-reared flies (Figure 2). When flies were exposed to the light, the Ktub proteins translocated from the rhabdomere to the cytoplasm of the photoreceptor cells, suggesting that the function of Ktub is light-dependent (Figure 2). In Drosophila eyes, the photosensitive protein Rh1 is localized in the rhabdomeric microvilli in dark-reared flies. When flies receive light stimulation, rhodopsin kinase phosphorylates the Rh1 at its C-terminus[51, 52] and then internalizes into the cytoplasm of the photoreceptor cells (Figure 5 and ). Arrestin 1 and arrestin 2 are required for Rh1 endocytosis upon light stimulation[37, 53]. In the arrestins mutant, Rh1 remains in the active stage in the phototransduction cascade. The consequence of active Rh1 accumulation causes photoreceptor cells to undergo light-dependent retinal degeneration. Previous studies have revealed massive Rh1 endocytosis in norpA mutants, and that the Rh1 endocytosis in norpA mutant is blocked by the shi ts mutant allele[38, 55]. The current study reveals that the formation of RLVs in ktub mutant is less than that in wild type upon light stimulation (Figure 5). The ktub/norpA double mutant also suppressed Rh1 endocytosis and retinal degeneration, further supporting that Ktub regulates Rh1 endocytosis (Figure 6). In mice, Dynamin-1 is colocalized with TULP1 in the outer plexiform layer and the inner segments of retina. Biochemical analysis demonstrates that Dynamin-1 binds directly to TULP1. Dynamin-1 is a major component of vesicle formation in receptor mediated endocytosis, synaptic vesicle recycling, and vesicle trafficking in and out of the trans-Golgi network[56–58]. This study shows that Ktub has a similar function to Dynamin-1 in blocking norpA-mediated massive Rh1 endocytosis. Although this study does not provide evidence showing a direct interaction between Ktub and Drosophila Dynamin, the idea that Ktub may bind to Drosophila Dynamin is consistent with the observation that Tub protein binds to Dynamin and regulates vesicle transport in mice. In addition, the TUB-1 protein in C. elegans controls fat storage through the RAB-7 mediated endocytosis pathway. Previous studies support the function of Ktub in mediating the endocytotic pathway. Further research should test whether Ktub also binds to Dynamin or whether it mediates the endocytotic pathway through Rab-7.
To determine which domain of Ktub protein is involved in endocytosis, this study uses Ktub deletion constructions in which either the N-terminus or C-terminus of Ktub protein was deleted. This study also tests which constructs failed to mediate Rh1 endocytosis in photoreceptor cells. Results show that the deletion of the C-terminus in the Ktub protein blocked light-induced Rh1 endocytosis (Figure 8). Structural analysis indicates that the Tubby C-terminal domain binds to double strand DNA and its N-terminal domain activates transcription. Previous studies have also shown an unconventional secretion of Tubby and Tulp1, indicating that they function as phagocytosis ligands for retinal pigment epithelium and macrophage phagocytosis[24, 25]. Further investigation reveals that Tubby and Tulp1 act as bridging molecules, as their N-terminal region functions as an MreTK- binding domain and the C-terminal region functions as a phagocytosis prey-binding domain. This study shows that the deletion of the Ktub C-terminus blocked Rh1 endocytosis, suggesting that the C-terminus may contain an important domain for mediating endocytosis. It is worth further investigation which specific peptide sequence is involved in mediating endocytosis. Because of the large fragment of deletion, it cannot be ruled out the possibility that the results from the deletion constructs are not physiological. Although the role of the C-terminus of Ktub protein in endocytosis is not completely clear, this study provides valuable information to further investigate the function of Ktub in endocytosis. Taken together, this study provides substantial support for the function of Drosophila ktub in mediating Rh1 endocytosis and retinal degeneration under light-dependent conditions.
This study examined the function of Drosophila ktub gene in phototransduction. Immunocytochemical studies showed the subcellular localization of Ktub in photoreceptor cells is light-dependent. In ktub mutants, the rhodopsin endocytosis is blocked under light stimulation. We also observed a retinal degeneration phenotype in ktub mutants. Using deletion constructs, we found the C-terminus of Ktub is required for Rh1 endocytosis. Taken together, these results delimit the novel function of Ktub in Rh1 endoytosis and retinal degeneration.
The authors thank Bloomington Stock Center, Szeged Drosophila Stock Center, and the Developmental Studies Hybridoma Bank for stocks and antibodies. We also thank H. Sun, C. Y. Tang, and C. P. Hu for their assistance in microinjection and antibody production respectively. This study was supported by the National Science Council, Taiwan, Republic of China for SSF (NSC 98-2311-B-029-002-MY3 and NSC 99-2632-B-029-001-MY3).
- Ronshaugen M, McGinnis N, Inglis D, Chou D, Zhao J, McGinnis W: Structure and expression patterns of Drosophila TULP and TUSP, members of the tubby-like gene family. Mech Dev. 2002, 117: 209-215. 10.1016/S0925-4773(02)00211-3.View ArticlePubMed
- Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, Ahringer J, Ruvkun G: Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature. 2003, 421: 268-272. 10.1038/nature01279.View ArticlePubMed
- Mukhopadhyay A, Pan X, Lambright DG, Tissenbaum HA: An endocytic pathway as a target of tubby for regulation of fat storage. EMBO Rep. 2007, 8: 931-938. 10.1038/sj.embor.7401055.PubMed CentralView ArticlePubMed
- Heikenwalder MF, Koritschoner NP, Pajer P, Chaboissier MC, Kurz SM, Briegel KJ, Bartunek P, Zenke M: Molecular cloning, expression and regulation of the avian tubby-like protein 1 (tulp1) gene. Gene. 2001, 273: 131-139. 10.1016/S0378-1119(01)00578-9.View ArticlePubMed
- Lai CP, Lee CL, Chen PH, Wu SH, Yang CC, Shaw JF: Molecular analyses of the Arabidopsis TUBBY-like protein gene family. Plant Physiol. 2004, 134: 1586-1597. 10.1104/pp.103.037820.PubMed CentralView ArticlePubMed
- Yang Z, Zhou Y, Wang X, Gu S, Yu J, Liang G, Yan C, Xu C: Genomewide comparative phylogenetic and molecular evolutionary analysis of tubby-like protein family in Arabidopsis, rice, and poplar. Genomics. 2008, 92: 246-253. 10.1016/j.ygeno.2008.06.001.View ArticlePubMed
- North MA, Naggert JK, Yan Y, Noben-Trauth K, Nishina PM: Molecular characterization of TUB, TULP1, and TULP2, members of the novel tubby gene family and their possible relation to ocular diseases. Proc Natl Acad Sci USA. 1997, 94: 3128-3133. 10.1073/pnas.94.7.3128.PubMed CentralView ArticlePubMed
- Nishina PM, North MA, Ikeda A, Yan Y, Naggert JK: Molecular characterization of a novel tubby gene family member, TULP3, in mouse and humans. Genomics. 1998, 54: 215-220. 10.1006/geno.1998.5567.View ArticlePubMed
- Kleyn PW, Fan W, Kovats SG, Lee JJ, Pulido JC, Wu Y, Berkemeier LR, Misumi DJ, Holmgren L, Charlat O, Woolf EA, Tayber O, Brody T, Shu P, Hawkins F, Kennedy B, Baldini L, Ebeling C, Alperin GD, Deeds J, Lakey ND, Culpepper J, Chen H, Glucksmann-Kuis MA, Carlson GA, Duyk GM, Moore KJ: Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family. Cell. 1996, 85: 281-290. 10.1016/S0092-8674(00)81104-6.View ArticlePubMed
- Noben-Trauth K, Naggert JK, North MA, Nishina PM: A candidate gene for the mouse mutation tubby. Nature. 1996, 380: 534-538. 10.1038/380534a0.View ArticlePubMed
- Ohlemiller KK, Hughes RM, Mosinger-Ogilvie J, Speck JD, Grosof DH, Silverman MS: Cochlear and retinal degeneration in the tubby mouse. Neuroreport. 1995, 6: 845-849. 10.1097/00001756-199504190-00005.View ArticlePubMed
- Hagstrom SA, Duyao M, North MA, Li T: Retinal degeneration in tulp1−/− mice: vesicular accumulation in the interphotoreceptor matrix. Invest Ophthalmol Vis Sci. 1999, 40: 2795-2802.PubMed
- Ikeda S, Shiva N, Ikeda A, Smith RS, Nusinowitz S, Yan G, Lin TR, Chu S, Heckenlively JR, North MA, Naggert JK, Nishina PM, Duyao MP: Retinal degeneration but not obesity is observed in null mutants of the tubby-like protein 1 gene. Hum Mol Genet. 2000, 9: 155-163. 10.1093/hmg/9.2.155.View ArticlePubMed
- Ikeda A, Ikeda S, Gridley T, Nishina PM, Naggert JK: Neural tube defects and neuroepithelial cell death in Tulp3 knockout mice. Hum Mol Genet. 2001, 10: 1325-1334. 10.1093/hmg/10.12.1325.View ArticlePubMed
- Hagstrom SA, North MA, Nishina PL, Berson EL, Dryja TP: Recessive mutations in the gene encoding the tubby-like protein TULP1 in patients with retinitis pigmentosa. Nat Genet. 1998, 18: 174-176. 10.1038/ng0298-174.View ArticlePubMed
- Gu S, Lennon A, Li Y, Lorenz B, Fossarello M, North M, Gal A, Wright A: Tubby-like protein-1 mutations in autosomal recessive retinitis pigmentosa. Lancet. 1998, 351: 1103-1104. 10.1016/S0140-6736(05)79384-3.View ArticlePubMed
- Banerjee P, Kleyn PW, Knowles JA, Lewis CA, Ross BM, Parano E, Kovats SG, Lee JJ, Penchaszadeh GK, Ott J, Jacobson SG, Gilliam TC: TULP1 mutation in two extended Dominican kindreds with autosomal recessive retinitis pigmentosa. Nat Genet. 1998, 18: 177-179. 10.1038/ng0298-177.View ArticlePubMed
- Boggon TJ, Shan WS, Santagata S, Myers SC, Shapiro L: Implication of tubby proteins as transcription factors by structure-based functional analysis. Science. 1999, 286: 2119-2125. 10.1126/science.286.5447.2119.View ArticlePubMed
- Carroll K, Gomez C, Shapiro L: Tubby proteins: the plot thickens. Nat Rev Mol Cell Biol. 2004, 5: 55-63. 10.1038/nrm1278.View ArticlePubMed
- Xi Q, Pauer GJ, Marmorstein AD, Crabb JW, Hagstrom SA: Tubby-like protein 1 (TULP1) interacts with F-actin in photoreceptor cells. Invest Ophthalmol Vis Sci. 2005, 46: 4754-4761. 10.1167/iovs.05-0693.PubMed CentralView ArticlePubMed
- Xi Q, Pauer GJ, Ball SL, Rayborn M, Hollyfield JG, Peachey NS, Crabb JW, Hagstrom SA: Interaction between the photoreceptor-specific tubby-like protein 1 and the neuronal-specific GTPase dynamin-1. Invest Ophthalmol Vis Sci. 2007, 48: 2837-2844. 10.1167/iovs.06-0059.PubMed CentralView ArticlePubMed
- Hagstrom SA, Adamian M, Scimeca M, Pawlyk BS, Yue G, Li T: A role for the Tubby-like protein 1 in rhodopsin transport. Invest Ophthalmol Vis Sci. 2001, 42: 1955-1962.PubMed
- Grossman GH, Pauer GJ, Narendra U, Peachey NS, Hagstrom SA: Early synaptic defects in tulp1−/− mice. Invest Ophthalmol Vis Sci. 2009, 50: 3074-3083. 10.1167/iovs.08-3190.PubMed CentralView ArticlePubMed
- Caberoy NB, Li W: Unconventional secretion of tubby and tubby-like protein 1. FEBS Lett. 2009, 583: 3057-3062. 10.1016/j.febslet.2009.08.015.PubMed CentralView ArticlePubMed
- Caberoy NB, Maiguel D, Kim Y, Li W: Identification of tubby and tubby-like protein 1 as eat-me signals by phage display. Exp Cell Res. 2010, 316: 245-257. 10.1016/j.yexcr.2009.10.008.PubMed CentralView ArticlePubMed
- Caberoy NB, Zhou Y, Li W: Tubby and tubby-like protein 1 are new MerTK ligands for phagocytosis. EMBO J. 2010, 29: 3898-3910. 10.1038/emboj.2010.265.PubMed CentralView ArticlePubMed
- Montell C: Visual transduction in Drosophila. Annu Rev Cell Dev Biol. 1999, 15: 231-268. 10.1146/annurev.cellbio.15.1.231.View ArticlePubMed
- Zuker CS: The biology of vision of Drosophila. Proc Natl Acad Sci USA. 1996, 93: 571-576. 10.1073/pnas.93.2.571.PubMed CentralView ArticlePubMed
- Shieh BH: Molecular genetics of retinal degeneration: a Drosophila perspective. Fly (Austin). 2011, 5: 356-368.View Article
- Montell C, Rubin GM: Molecular characterization of the Drosophila trp locus: a putative integral membrane protein required for phototransduction. Neuron. 1989, 2: 1313-1323. 10.1016/0896-6273(89)90069-X.View ArticlePubMed
- Van Vactor D, O'Reilly AM, Neel BG: Genetic analysis of protein tyrosine phosphatases. Curr Opin Genet Dev. 1998, 8: 112-126. 10.1016/S0959-437X(98)80070-1.View ArticlePubMed
- Kumar JP, Ready DF: Rhodopsin plays an essential structural role in Drosophila photoreceptor development. Development. 1995, 121: 4359-4370.PubMed
- Bennett N, Sitaramayya A: Inactivation of photoexcited rhodopsin in retinal rods: the roles of rhodopsin kinase and 48-kDa protein (arrestin). Biochemistry. 1988, 27: 1710-1715. 10.1021/bi00405a049.View ArticlePubMed
- Kuhn H, Wilden U: Deactivation of photoactivated rhodopsin by rhodopsin-kinase and arrestin. J Recept Res. 1987, 7: 283-298.PubMed
- Hardie RC, Raghu P: Visual transduction in Drosophila. Nature. 2001, 413: 186-1893. 10.1038/35093002.View ArticlePubMed
- Laporte SA, Oakley RH, Holt JA, Barak LS, Caron MG: The interaction of beta-arrestin with the AP-2 adaptor is required for the clustering of beta 2-adrenergic receptor into clathrin-coated pits. J Biol Chem. 2000, 275: 23120-23126. 10.1074/jbc.M002581200.View ArticlePubMed
- Satoh AK, Ready DF: Arrestin1 mediates light-dependent rhodopsin endocytosis and cell survival. Curr Biol. 2005, 15: 1722-1733. 10.1016/j.cub.2005.08.064.View ArticlePubMed
- Alloway PG, Howard L, Dolph PJ: The formation of stable rhodopsin-arrestin complexes induces apoptosis and photoreceptor cell degeneration. Neuron. 2000, 28: 129-138. 10.1016/S0896-6273(00)00091-X.View ArticlePubMed
- Kiselev A, Socolich M, Vinos J, Hardy RW, Zuker CS, Ranganathan R: A molecular pathway for light-dependent photoreceptor apoptosis in Drosophila. Neuron. 2000, 28: 139-152. 10.1016/S0896-6273(00)00092-1.View ArticlePubMed
- Lee SJ, Xu H, Kang LW, Amzel LM, Montell C: Light adaptation through phosphoinositide-regulated translocation of Drosophila visual arrestin. Neuron. 2003, 39: 121-132. 10.1016/S0896-6273(03)00390-8.View ArticlePubMed
- Yao JG, Sun YH: Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development. EMBO J. 2005, 24: 2602-2612. 10.1038/sj.emboj.7600725.PubMed CentralView ArticlePubMed
- Spradling AC, Rubin GM: Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982, 218: 341-347. 10.1126/science.6289435.View ArticlePubMed
- Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979, 76: 4350-4354. 10.1073/pnas.76.9.4350.PubMed CentralView ArticlePubMed
- Fan SS: Dynactin affects extension and assembly of adherens junctions in Drosophila photoreceptor development. J Biomed Sci. 2004, 11: 362-369.PubMed
- Lewis CA, Batlle IR, Batlle KG, Banerjee P, Cideciyan AV, Huang J, Aleman TS, Huang Y, Ott J, Gilliam TC, Knowles JA, Jacobson SG: Tubby-like protein 1 homozygous splice-site mutation causes early-onset severe retinal degeneration. Invest Ophthalmol Vis Sci. 1999, 40: 2106-2114.PubMed
- Ikeda A, Zheng QY, Rosenstiel P, Maddatu T, Zuberi AR, Roopenian DC, North MA, Naggert JK, Johnson KR, Nishina PM: Genetic modification of hearing in tubby mice: evidence for the existence of a major gene (moth1) which protects tubby mice from hearing loss. Hum Mol Genet. 1999, 8: 1761-1767. 10.1093/hmg/8.9.1761.PubMed CentralView ArticlePubMed
- Kong L, Li F, Soleman CE, Li S, Elias RV, Zhou X, Lewis DA, McGinnis JF, Cao W: Bright cyclic light accelerates photoreceptor cell degeneration in tubby mice. Neurobiol Dis. 2006, 21: 468-477. 10.1016/j.nbd.2005.08.017.View ArticlePubMed
- Acharya U, Patel S, Koundakjian E, Nagashima K, Han X, Acharya JK: Modulating sphingolipid biosynthetic pathway rescues photoreceptor degeneration. Science. 2003, 299: 1740-1743. 10.1126/science.1080549.View ArticlePubMed
- Orem NR, Dolph PJ: Epitope masking of rhabdomeric rhodopsin during endocytosis-induced retinal degeneration. Mol Vis. 2002, 8: 455-461.PubMed
- Hsu CD, Whaley MA, Frazer K, Miller DA, Mitchell KA, Adams SM, O'Tousa JE: Limited role of developmental programmed cell death pathways in Drosophila norpA retinal degeneration. J Neurosci. 2004, 24: 500-507. 10.1523/JNEUROSCI.3328-02.2004.View ArticlePubMed
- Vinos J, Jalink K, Hardy RW, Britt SG, Zuker CS: A G protein-coupled receptor phosphatase required for rhodopsin function. Science. 1997, 277: 687-690. 10.1126/science.277.5326.687.View ArticlePubMed
- Lee SJ, Xu H, Montell C: Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila. Proc Natl Acad Sci USA. 2004, 101: 11874-11879. 10.1073/pnas.0402205101.PubMed CentralView ArticlePubMed
- Orem NR, Xia L, Dolph PJ: An essential role for endocytosis of rhodopsin through interaction of visual arrestin with the AP-2 adaptor. J Cell Sci. 2006, 119: 3141-3148. 10.1242/jcs.03052.View ArticlePubMed
- Dolph PJ, Ranganathan R, Colley NJ, Hardy RW, Socolich M, Zuker CS: Arrestin function in inactivation of G protein-coupled receptor rhodopsin in vivo. Science. 1993, 260: 1910-1916. 10.1126/science.8316831.View ArticlePubMed
- Orem NR, Dolph PJ: Loss of the phospholipase C gene product induces massive endocytosis of rhodopsin and arrestin in Drosophila photoreceptors. Vision Res. 2002, 42: 497-505. 10.1016/S0042-6989(01)00229-2.View ArticlePubMed
- Van der Bliek AM: Functional diversity in the dynamin family. Trends Cell Biol. 1999, 9: 96-102. 10.1016/S0962-8924(98)01490-1.View ArticlePubMed
- McNiven MA, Kim L, Krueger EW, Orth JD, Cao H, Wong TW: Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape. J Cell Biol. 2000, 151: 187-198. 10.1083/jcb.151.1.187.PubMed CentralView ArticlePubMed
- Tomizawa K, Sunada S, Lu YF, Oda Y, Kinuta M, Ohshima T, Saito T, Wei FY, Matsushita M, Li ST, Tsutsui K, Hisanaga S, Mikoshiba K, Takei K, Matsui H: Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles. J Cell Biol. 2003, 163: 813-824. 10.1083/jcb.200308110.PubMed CentralView ArticlePubMed
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