N-ethyl-N-nitrosourea mutagenesis produced a small number of mice with altered plasma electrolyte levels

Table 3 Mutant lines showing deviations of plasma electrolyte values derived from the Munich ENU mouse mutagenesis project

				Altered plasma electrolyte values^b: total (m/f)
Phenotype	Mode of inheritance	Line	Offspring tested^a: n (m/f)	n	% frequency	Mean (mmol/l)
Ca low	D	CA001	6 (2/4)	3 (2/1)	50 (100/25)	1.8/1.8
K high	D	KAL006	45 (26/19)	13 (10/3)	29 (38/16)	6.1/6.2
K high	R	KAL003	21 (14/7)	7 (5/2)	33 (36/29)	6.1/5.9
K high	R	KAL004	10 (5/5)	4 (2/2)	40 (40/40)	6.4/6.6
K high	R	KAL007	10 (5/5)	4 (3/1)	40 (60/20)	7.5/7.4
			10 (6/4)	6 (2/4)	60 (33/100)	7.6/7.3

m/f, males/females; D, dominant mutation; R, recessive mutation.
^a Offspring derived from mating heterozygous phenotypic mutants to wild-type mice (screen for dominant mutations) and after breeding homozygous mutants to heterozygous mutant mice (screen for recessive mutations). In addition, line KAL007 was successfully bred by mating homozygous mutants (second line).
^b The frequency of the altered phenotype in the offspring was expected to be 50% after mating heterozygous phenotypic mutants to wild-type mice (screen for dominant mutations) and after breeding homozygous mutants to heterozygous mutant mice (screen for recessive mutations). All offspring were expected to harbor the altered phenotype after breeding homozygous mutants of line KAL007 (second line). The absolute numbers of observed phenotypic mutants are given (n).

ISSN: 1423-0127