Figure 2

Progression and regression of LNCaP 104-R1 tumor xenografts in nude mice treated with testosterone. (A) LNCaP 104-R1 tumor xenografts in castrated male nude mice were allowed to grow until they reached an average volume of 300 mm³ on the 58th day. On the 67th day, mice were separated into a control group (open circles) and a treatment group (filled circles). The treatment group received a subcutaneous implant of a 20 mg testosterone pellet. The mice in the control group were implanted with a 20 mg testosterone pellet on day 121. Open circles represent tumor in mice without testosterone, while filled circles and filled squares represent tumors in mice with testosterone. Tumor volumes are expressed as the mean ± standard error [15]. (B) For mice carrying adapted R1Ad tumors from (A), testosterone pellets were removed from 5 mice (10 tumors). Their tumor growth was compared with tumors in mice bearing testosterone pellets (5 mice with 10 tumors) [15]. (C) PSA, AR, and actin protein levels in 104-S tumor (in intact mice), 104-R1-T tumors, R1Ad-1+T tumors, and R1Ad-T were assayed by Western blot [15]. (D) Serum PSA level of mice with 104-S tumors (in intact mice), 104-R1-T tumors, 104-R1+T tumors, R1Ad+T tumors, R1Ad-T tumors was determined by ELISA [15].