Figure 5From: Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagyInduction of autophagy by reversine. (A) OC2 cells were treated without (DMSO) or with reversine (5 and 10 μM) for 24 h. Phase-contrast microscopy were taken with 200× magnification. Autophagic vesicles were observed in cells treated with reversine. (B) OSCC cells were pre-treated with 1 or 3 mM 3-MA for 2 hours, followed by treatment without or with 10 μM reversine. At indicated time points, cells were harvested for analyzing the cytoplasmic form LC3-I or the membrane form LC3-II. (C) OCSL cells were transfected with vectors expressing either GFP or GFP-LC3-II, followed by treatment of 3-MA and/or reversine as described. OSCL cells were analyzed under confocal microscope. (D) The same as (C) except that two different concentrations of 3-MA were evaluated. The percentages of cells with puncta were calculated by examining over 100 GFP positive cells. All values were expressed as means ± SD of three independent experiments, each of which was done in triplicate.Back to article page