Fig. 1

Effects of sulforaphane on the cell viability and clonogenic ability of NSCLC cells. (a) Time- and concentration-dependent inhibition of NSCLC cell viability by sulforaphane (SFN). Cells were treated with various concentrations of sulforaphane for 24 h (upper panel), 48 h (middle panel) and 72 h (lower panel), and cell viability was evaluated by MTT assay. (b) Inhibition of the clonogenic ability of NSCLC cells by sulforaphane. H1975 (upper panel), PC9/gef (middle panel) and A549 (lower panel) cells were treated with various concentrations of sulforaphane for 6 days and cultured for an additional 8 days in the absence of sulforaphane. The numbers of foci were scored, and the data are presented as relative foci-forming ability (FFA). Data are expressed as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; and ***p < 0.001, as analyzed with the unpaired t-test