Fig. 6
Inhibitory effects of PD98059 and SB203580 on MCP-1 secretion and cell adhesion functions in EA. hy926 cells. EA. hy926 cells (1 × 106/mL) were pretreated with PD98059 (25 μM), SB203580 (10 μM), or PD98059 (25 μM) and SB203580 (10 μM) for 30 min, after which they were stimulated with LPS (1 μg/mL) for 24 h and with trypsin (5 μg/mL) for 12 h. Control cells were treated with 0.1% DMSO. a MCP-1 protein levels were quantified through immunoblotting, and β-actin was used as the loading control. The upper panel shows a typical blot. Bar graphs represent means ± SEM from four independent experiments. #p < 0.05 compared with the trypsin treatment only group; *p < 0.05 compared with the trypsin plus LPS-pretreatment group; **p < 0.01 compared with the trypsin plus LPS-pretreatment group b Effects of PD98059 and SB203580 on trypsin-induced mononuclear cell–EC adhesion in LPS-pretreated ECs. Microphotographs (100×) show the adhesion of mononuclear cells to ECs treated as described previously. c The adhesion assay was performed using mononuclear cells. Bar graphs represent means ± SEM from four independent experiments. #p < 0.05 compared with the trypsin treatment only group; *p < 0.05 compared with the trypsin plus LPS-pretreatment group